ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLSYYEHNTA FQTNNCNSGS NAATTYNSDA NNDTIMNKRK NDHFEFDTHT FYQRSKRTKR 

        70         80         90        100        110        120 
DSVSTKFSVG SGCANLNNNN NNIIINNNNN NNNNNNNHNH NNSNNTATYN NIHYKKNIEI 

       130        140        150        160        170        180 
CPLKPVSMHH TMNSRLLNES EFYSETEEYM IHGYFGNTNR DITGTSPTGS ASIIQHQYHL 

       190        200        210 
LPSQSIIASQ APGTAMAALT NNNIANDYMD ID 

References and documentation are available.

Number of amino acids: 212

Molecular weight: 23942.85

Theoretical pI: 6.39

Amino acid composition: 
Ala (A)  13	  6.1%
Arg (R)   6	  2.8%
Asn (N)  45	 21.2%
Asp (D)   9	  4.2%
Cys (C)   3	  1.4%
Gln (Q)   6	  2.8%
Glu (E)   8	  3.8%
Gly (G)   8	  3.8%
His (H)  11	  5.2%
Ile (I)  15	  7.1%
Leu (L)   8	  3.8%
Lys (K)   8	  3.8%
Met (M)   7	  3.3%
Phe (F)   7	  3.3%
Pro (P)   5	  2.4%
Ser (S)  20	  9.4%
Thr (T)  19	  9.0%
Trp (W)   0	  0.0%
Tyr (Y)  11	  5.2%
Val (V)   3	  1.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 17
Total number of positively charged residues (Arg + Lys): 14

Atomic composition:

Carbon      C	      1011
Hydrogen    H	      1543
Nitrogen    N	       311
Oxygen      O	       348
Sulfur      S	        10

Formula: C1011H1543N311O348S10
Total number of atoms: 3223

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16515
Abs 0.1% (=1 g/l)   0.690, assuming all pairs of Cys residues form cystines


Ext. coefficient    16390
Abs 0.1% (=1 g/l)   0.685, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.74
This classifies the protein as unstable.



Aliphatic index: 52.55

Grand average of hydropathicity (GRAVY): -1.000