ProtParam
User-provided sequence:
10 20 30 40 50 60
MLTDEREVVC VTGASGCIGS WLVHQLLLRG YSVHATVKNL QDEKETKHLE GLEGAATRLH
70 80 90 100 110 120
LFEMDLLQYD TVSAAINGCS GVFHLASPCI VDEVQDPQKQ LLDPAVKGTI NVLTAAKEAS
130 140 150 160 170 180
VKRVVVTSSI SAITPSPNWP ADKIKNEECW AAEDYCRQNG LWYPLSKTLA EKAAWEFAEE
190 200 210 220 230 240
KGLDVVVVNP GTVMGPVIPP SLNASMHMLL RLLQGCTETY ENFFMGSVHF KDVALAHILV
250 260 270 280 290 300
YEDPYSKGRH LCVEAISHYG DFVAKVAELY PNYNVPKLPR ETQPGLLRDK NASKKLIDLG
310 320
LKFISMEEII KEGVESLKSK GFIS
References and
documentation are available.
Number of amino acids: 324
Molecular weight: 35757.16
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1602
Hydrogen H 2532
Nitrogen N 420
Oxygen O 475
Sulfur S 15
Formula: C
1602H
2532N
420O
475S
15
Total number of atoms: 5044
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42900
Abs 0.1% (=1 g/l) 1.200, assuming all pairs of Cys residues form cystines
Ext. coefficient 42400
Abs 0.1% (=1 g/l) 1.186, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.17
This classifies the protein as stable.
Aliphatic index: 96.27
Grand average of hydropathicity (GRAVY): -0.072