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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLTDEREVVC VTGASGCIGS WLVHQLLLRG YSVHATVKNL QDEKETKHLE GLEGAATRLH

70 80 90 100 110 120
LFEMDLLQYD TVSAAINGCS GVFHLASPCI VDEVQDPQKQ LLDPAVKGTI NVLTAAKEAS

130 140 150 160 170 180
VKRVVVTSSI SAITPSPNWP ADKIKNEECW AAEDYCRQNG LWYPLSKTLA EKAAWEFAEE

190 200 210 220 230 240
KGLDVVVVNP GTVMGPVIPP SLNASMHMLL RLLQGCTETY ENFFMGSVHF KDVALAHILV

250 260 270 280 290 300
YEDPYSKGRH LCVEAISHYG DFVAKVAELY PNYNVPKLPR ETQPGLLRDK NASKKLIDLG

310 320
LKFISMEEII KEGVESLKSK GFIS


References and documentation are available.

Number of amino acids: 324 Molecular weight: 35757.16 Theoretical pI: 5.62
Amino acid composition: 
Ala (A) 26 8.0% Arg (R) 9 2.8% Asn (N) 12 3.7% Asp (D) 15 4.6% Cys (C) 8 2.5% Gln (Q) 9 2.8% Glu (E) 26 8.0% Gly (G) 21 6.5% His (H) 10 3.1% Ile (I) 15 4.6% Leu (L) 36 11.1% Lys (K) 23 7.1% Met (M) 7 2.2% Phe (F) 9 2.8% Pro (P) 16 4.9% Ser (S) 22 6.8% Thr (T) 15 4.6% Trp (W) 5 1.5% Tyr (Y) 10 3.1% Val (V) 30 9.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 41 Total number of positively charged residues (Arg + Lys): 32 Atomic composition: Carbon C 1602 Hydrogen H 2532 Nitrogen N 420 Oxygen O 475 Sulfur S 15 Formula: C1602H2532N420O475S15 Total number of atoms: 5044 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 42900 Abs 0.1% (=1 g/l) 1.200, assuming all pairs of Cys residues form cystines Ext. coefficient 42400 Abs 0.1% (=1 g/l) 1.186, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 31.17 This classifies the protein as stable. Aliphatic index: 96.27 Grand average of hydropathicity (GRAVY): -0.072