ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLTITKRLVT TDVRSRILLS SLNGKMSDAL ALLRQQQQTS VDVELLHTML ARAAALAHAD 

        70         80         90        100        110        120 
TIAYMWYQHV MPRRLPVEGR LLCEMAGVAL YQDRLFLPAQ FLQHYQAMNR DRRTSPEDEL 

       130        140        150        160        170        180 
IEYELRRIKV EAFARGTMHS TALREKWKVF LQEMDTLPGQ PPLRLRDFPQ MTKAMGIALM 

       190        200        210        220        230        240 
QQDEQAAALA LFGRQPLVIK NEWSLPLLLA GVLWHVPGPA QARRVLAEFR QSYRGLPLLD 

       250 
AELVIKRRGF EINT 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 29155.14

Theoretical pI: 9.75

Amino acid composition: 
Ala (A)  27	 10.6%
Arg (R)  25	  9.8%
Asn (N)   4	  1.6%
Asp (D)  11	  4.3%
Cys (C)   1	  0.4%
Gln (Q)  18	  7.1%
Glu (E)  15	  5.9%
Gly (G)  11	  4.3%
His (H)   6	  2.4%
Ile (I)   9	  3.5%
Leu (L)  40	 15.7%
Lys (K)   8	  3.1%
Met (M)  12	  4.7%
Phe (F)   8	  3.1%
Pro (P)  13	  5.1%
Ser (S)   9	  3.5%
Thr (T)  13	  5.1%
Trp (W)   4	  1.6%
Tyr (Y)   6	  2.4%
Val (V)  14	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 33

Atomic composition:

Carbon      C	      1303
Hydrogen    H	      2107
Nitrogen    N	       375
Oxygen      O	       357
Sulfur      S	        13

Formula: C1303H2107N375O357S13
Total number of atoms: 4155

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30940
Abs 0.1% (=1 g/l)   1.061, assuming all pairs of Cys residues form cystines


Ext. coefficient    30940
Abs 0.1% (=1 g/l)   1.061, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.20
This classifies the protein as unstable.



Aliphatic index: 101.85

Grand average of hydropathicity (GRAVY): -0.143