ProtParam
User-provided sequence:
10 20 30 40 50 60
MLTSILIKGR TPVLASRNLL AALSNCNHIT WAVSRRLYND GNRDQRNFGR NQRNNNSNRY
70 80 90 100 110 120
RNSRFNSRPR TRSREDDDEV HFDKTTFSKL IHVPKEDNSK EVTLDSLLEE GVLDKEIHKA
130 140 150 160 170 180
ITRMEFPGLT PVQQKTIKPI LSSEDHDVIA RAKTGTGKTF AFLIPIFQHL INTKFDSQYM
190 200 210 220 230 240
VKAVIVAPTR DLALQIEAEV KKIHDMNYGL KKYACVSLVG GTDFRAAMNK MNKLRPNIVI
250 260 270 280 290 300
ATPGRLIDVL EKYSNKFFRF VDYKVLDEAD RLLEIGFRDD LETISGILNE KNSKSADNIK
310 320 330 340 350 360
TLLFSATLDD KVQKLANNIM NKKECLFLDT VDKNEPEAHE RIDQSVVISE KFANSIFAAV
370 380 390 400 410 420
EHIKKQIKER DSNYKAIIFA PTVKFTSFLC SILKNEFKKD LPILEFHGKI TQNKRTSLVK
430 440 450 460 470 480
RFKKDESGIL VCTDVGARGM DFPNVHEVLQ IGVPSELANY IHRIGRTARS GKEGSSVLFI
490 500 510 520 530 540
CKDELPFVRE LEDAKNIVIA KQEKYEPSEE IKSEVLEAVT EEPEDISDIV ISLISSYRSC
550 560 570 580 590 600
IKEYRFSERR ILPEIASTYG VLLNDPQLKI PVSRRFLDKL GLSRSPIGKA MFEIRDYSSR
610 620 630 640 650 660
DGNNKSYDYD DDSEISFRGN KNYNNRSQNR DYDDEPFRRS NNNRRSFSRS NDKNNYSSRN
SNIY
References and
documentation are available.
Number of amino acids: 664
Molecular weight: 76268.61
Theoretical pI: 9.07
Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 108
Atomic composition:
Carbon C 3364
Hydrogen H 5390
Nitrogen N 964
Oxygen O 1026
Sulfur S 16
Formula: C
3364H
5390N
964O
1026S
16
Total number of atoms: 10760
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 35675
Abs 0.1% (=1 g/l) 0.468, assuming all pairs of Cys residues form cystines
Ext. coefficient 35300
Abs 0.1% (=1 g/l) 0.463, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 51.71
This classifies the protein as unstable.
Aliphatic index: 84.56
Grand average of hydropathicity (GRAVY): -0.600