ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLVFKRGIHV VPKLPNSKAL LQNGVPNILS SSGFKTVWFD YQRYLCDKLT LATAGQSLES 

        70         80         90        100        110        120 
YYPFHILLKT AGNPLQSNIF NLASSIHNNH LFVENILPSA VEHGTNSNAV VKTEPSRLFL 

       130        140        150        160        170        180 
SKIKDSFNGS DWEVVKEEMI YRAENEVLGQ GWLFLVENNE KKLFILTSNN NGTPYYFPRN 

       190        200        210        220        230        240 
QSFDLNSAIS IDEFATLKQM KELIGKSTKL NGKVQDWTMP IICVNLWDHA YLHDYGVGNR 

       250        260 
SKYVKNVLDN LNWSVVNNRI FSGISK 

References and documentation are available.

Number of amino acids: 266

Molecular weight: 30223.53

Theoretical pI: 8.89

Amino acid composition: 
Ala (A)  11	  4.1%
Arg (R)   7	  2.6%
Asn (N)  28	 10.5%
Asp (D)  10	  3.8%
Cys (C)   2	  0.8%
Gln (Q)   8	  3.0%
Glu (E)  13	  4.9%
Gly (G)  15	  5.6%
His (H)   7	  2.6%
Ile (I)  16	  6.0%
Leu (L)  30	 11.3%
Lys (K)  20	  7.5%
Met (M)   4	  1.5%
Phe (F)  14	  5.3%
Pro (P)  10	  3.8%
Ser (S)  24	  9.0%
Thr (T)  11	  4.1%
Trp (W)   6	  2.3%
Tyr (Y)  10	  3.8%
Val (V)  20	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1374
Hydrogen    H	      2123
Nitrogen    N	       363
Oxygen      O	       394
Sulfur      S	         6

Formula: C1374H2123N363O394S6
Total number of atoms: 4260

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48025
Abs 0.1% (=1 g/l)   1.589, assuming all pairs of Cys residues form cystines


Ext. coefficient    47900
Abs 0.1% (=1 g/l)   1.585, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.48
This classifies the protein as stable.



Aliphatic index: 93.38

Grand average of hydropathicity (GRAVY): -0.241