ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLVFTTSPDH VDELNEFVQQ LNPVAFTRVL RGLGKVLASY NDKAVEEDTL KKSSTGSLPS 

        70         80         90        100        110        120 
GQQVHCQYVL DDPNHVEGIS VDQSLQVPKF EKNWLISPPG SPPVGWEPIV EESPNSQHLA 

       130        140        150        160 
HDIQLKLDEL GNALLNDHSA GPQIVISEHN NTKETSPSRQ FEH 

References and documentation are available.

Number of amino acids: 163

Molecular weight: 18051.05

Theoretical pI: 4.91

Amino acid composition: 
Ala (A)   6	  3.7%
Arg (R)   3	  1.8%
Asn (N)  10	  6.1%
Asp (D)  10	  6.1%
Cys (C)   1	  0.6%
Gln (Q)  11	  6.7%
Glu (E)  13	  8.0%
Gly (G)   9	  5.5%
His (H)   8	  4.9%
Ile (I)   6	  3.7%
Leu (L)  17	 10.4%
Lys (K)   8	  4.9%
Met (M)   1	  0.6%
Phe (F)   5	  3.1%
Pro (P)  13	  8.0%
Ser (S)  16	  9.8%
Thr (T)   7	  4.3%
Trp (W)   2	  1.2%
Tyr (Y)   2	  1.2%
Val (V)  15	  9.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 11

Atomic composition:

Carbon      C	       797
Hydrogen    H	      1241
Nitrogen    N	       219
Oxygen      O	       256
Sulfur      S	         2

Formula: C797H1241N219O256S2
Total number of atoms: 2515

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.774, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.774, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.62
This classifies the protein as unstable.



Aliphatic index: 85.40

Grand average of hydropathicity (GRAVY): -0.534