ProtParam
User-provided sequence:
10 20 30 40 50 60
MLVLGSLRSA LSCSSTASLI SKRNPCYPYG ILCRTLSQSV KLWQENTSKD DSSLNITPRL
70 80 90 100 110 120
LKIIPNDTDI VTLEKQDELI KRRRKLSKEV TQMKRLKPVS PGLRWYRSPI YPYLYKGRPV
130 140 150 160 170 180
RALTVVRKKH GGRNNSGKIT VRHQGGGHRN RTRLIDFNRW EGGAQTVQRI EYDPGRSSHI
190 200 210 220 230 240
ALLKHNTTGE LSYIIACDGL RPGDVVESFR RGIPQTLLNE MGGKVDPAIL SVKTTQRGNC
250 260 270 280 290 300
LPISMIPIGT IIHNVGITPV GPGKFCRSAG TYARVLAKLP EKKKAIVRLQ SGEHRYVSLE
310 320 330 340 350 360
AVATIGVVSN IDHQNRSLGK AGRSRWLGIR PTVRGVAMNK CDHPHGGGRG KSKSNKLSMS
370 380 390
PWGQLAKGYK TRRGKNQNRM KVKDRPRGKD ARL
References and
documentation are available.
Number of amino acids: 393
Molecular weight: 43785.85
Theoretical pI: 10.78
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 74
Atomic composition:
Carbon C 1914
Hydrogen H 3177
Nitrogen N 605
Oxygen O 542
Sulfur S 14
Formula: C
1914H
3177N
605O
542S
14
Total number of atoms: 6252
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44265
Abs 0.1% (=1 g/l) 1.011, assuming all pairs of Cys residues form cystines
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 1.002, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.05
This classifies the protein as unstable.
Aliphatic index: 84.30
Grand average of hydropathicity (GRAVY): -0.577