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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLVLGSLRSA LSCSSTASLI SKRNPCYPYG ILCRTLSQSV KLWQENTSKD DSSLNITPRL

70 80 90 100 110 120
LKIIPNDTDI VTLEKQDELI KRRRKLSKEV TQMKRLKPVS PGLRWYRSPI YPYLYKGRPV

130 140 150 160 170 180
RALTVVRKKH GGRNNSGKIT VRHQGGGHRN RTRLIDFNRW EGGAQTVQRI EYDPGRSSHI

190 200 210 220 230 240
ALLKHNTTGE LSYIIACDGL RPGDVVESFR RGIPQTLLNE MGGKVDPAIL SVKTTQRGNC

250 260 270 280 290 300
LPISMIPIGT IIHNVGITPV GPGKFCRSAG TYARVLAKLP EKKKAIVRLQ SGEHRYVSLE

310 320 330 340 350 360
AVATIGVVSN IDHQNRSLGK AGRSRWLGIR PTVRGVAMNK CDHPHGGGRG KSKSNKLSMS

370 380 390
PWGQLAKGYK TRRGKNQNRM KVKDRPRGKD ARL


References and documentation are available.

Number of amino acids: 393 Molecular weight: 43785.85 Theoretical pI: 10.78
Amino acid composition: 
Ala (A) 17 4.3% Arg (R) 41 10.4% Asn (N) 18 4.6% Asp (D) 14 3.6% Cys (C) 7 1.8% Gln (Q) 13 3.3% Glu (E) 12 3.1% Gly (G) 39 9.9% His (H) 10 2.5% Ile (I) 26 6.6% Leu (L) 36 9.2% Lys (K) 33 8.4% Met (M) 7 1.8% Phe (F) 3 0.8% Pro (P) 22 5.6% Ser (S) 32 8.1% Thr (T) 22 5.6% Trp (W) 5 1.3% Tyr (Y) 11 2.8% Val (V) 25 6.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 26 Total number of positively charged residues (Arg + Lys): 74 Atomic composition: Carbon C 1914 Hydrogen H 3177 Nitrogen N 605 Oxygen O 542 Sulfur S 14 Formula: C1914H3177N605O542S14 Total number of atoms: 6252 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 44265 Abs 0.1% (=1 g/l) 1.011, assuming all pairs of Cys residues form cystines Ext. coefficient 43890 Abs 0.1% (=1 g/l) 1.002, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 52.05 This classifies the protein as unstable. Aliphatic index: 84.30 Grand average of hydropathicity (GRAVY): -0.577