ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLVPPANFGI AEEGIYRCSK VETLNLSFLE TLNLKTAIFI GGQEPSKFFK DFFTRSSIKW 

        70         80         90        100        110        120 
IVLRMSDFSA AAVPVKSSSV SNANLYSNNN STLSLQEEKK KSTANGSQNS TTGDPVIQEE 

       130        140        150        160        170        180 
LAYHLTDNDD LMLIKSTCLK RTFKTLLNVD NYNVLLVDKT ALVIGILRKI QKWNIASIIN 

       190        200        210        220        230        240 
EYRLFSGKNR NYFAETFLEI INIEIEQEKD NKTIVDNKAK KLPLENNRTH SIEYKANSGK 

       250        260        270        280        290        300 
LIRVNEDDLC REPEVPQRLL TLINQIETKV KNNKVLQVSG VLGDDLKKTS SDLGIFGHRY 

       310        320        330        340        350        360 
RLAFNKKENG DYGYYKARGK DNVKIRIPCD SELPDWFRFQ RDLWEKENVP EEHHFYREHI 


FT 

References and documentation are available.

Number of amino acids: 362

Molecular weight: 41647.43

Theoretical pI: 8.62

Amino acid composition: 
Ala (A)  16	  4.4%
Arg (R)  18	  5.0%
Asn (N)  32	  8.8%
Asp (D)  20	  5.5%
Cys (C)   4	  1.1%
Gln (Q)  10	  2.8%
Glu (E)  28	  7.7%
Gly (G)  16	  4.4%
His (H)   6	  1.7%
Ile (I)  27	  7.5%
Leu (L)  37	 10.2%
Lys (K)  34	  9.4%
Met (M)   3	  0.8%
Phe (F)  18	  5.0%
Pro (P)  11	  3.0%
Ser (S)  26	  7.2%
Thr (T)  20	  5.5%
Trp (W)   4	  1.1%
Tyr (Y)  12	  3.3%
Val (V)  20	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 48
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      1864
Hydrogen    H	      2952
Nitrogen    N	       508
Oxygen      O	       559
Sulfur      S	         7

Formula: C1864H2952N508O559S7
Total number of atoms: 5890

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40130
Abs 0.1% (=1 g/l)   0.964, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.958, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.04
This classifies the protein as stable.



Aliphatic index: 89.39

Grand average of hydropathicity (GRAVY): -0.510