ProtParam
User-provided sequence:
10 20 30 40 50 60
MLVVGMHVEI KRSDGRIHGA VIAEVKSNGR FMVEWYEKGE TKGKESSLEE LLTLNPSLQA
70 80 90 100 110 120
PKPTPPPQPP QKTLQASTAV NRQNGIHAQS MILDDEDTFL LDHINMIPAK GASANKRPTG
130 140 150 160 170 180
AFTQHVPVRM APPSEKPLPT RRAPSPKEDA APAPKSTRTT AAFKPDLDST AITVPKHTAR
190 200 210 220 230 240
RTVVVAPAPA PVPAPPIANM TSQRAPSPVA RVPSPKNVPR SYPQQDVQPS NGNFAFAEMI
250 260 270 280 290 300
RNYRAQIDYR PLSMFDGVNE NRISVCVRKR PLNKKELTKA EVDVITIPSR DITILHQPQT
310 320 330 340 350 360
RVDLTKYLDN QKFRFDYSFD EYANNELVYR FTAAPLVKTV FDNGTATCFA YGQTGSGKTH
370 380 390 400 410 420
TMGGDFSGKK QNASMGIYAL TARDVFRMLE QPQYRRKDLS VHCAFFEIYG TKTYDLLNDK
430 440 450 460 470 480
AELRVLEDKM QKVQVVGLKE EQAHNEQDVL ELINKGTLVR TAGTTSANAN SSRSHAIFQI
490 500 510 520 530 540
ILRQGKKVWG KFSLIDLAGN ERGQDTRECD RDTRKEGANI NTSLLALKEC IRGMARNSSH
550 560 570 580 590 600
VPFRQSKLTM VLRDSFIGEK SRTVMISMIS PGISSSDHTL NTLRYADRVK EMGTDGSGEA
610 620 630 640 650 660
TPIRDEELFL PPSADKSDEE YDEMVEKQEH RRVAVDHVRN LKDMSEKIIR ETTIVLSNEP
670 680
SAAQKAECLA KLDQLAQIVS NTRVAVENV
References and
documentation are available.
Number of amino acids: 689
Molecular weight: 76717.22
Theoretical pI: 9.09
Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 95
Atomic composition:
Carbon C 3348
Hydrogen H 5402
Nitrogen N 978
Oxygen O 1033
Sulfur S 26
Formula: C
3348H
5402N
978O
1033S
26
Total number of atoms: 10787
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33725
Abs 0.1% (=1 g/l) 0.440, assuming all pairs of Cys residues form cystines
Ext. coefficient 33350
Abs 0.1% (=1 g/l) 0.435, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.96
This classifies the protein as unstable.
Aliphatic index: 75.60
Grand average of hydropathicity (GRAVY): -0.558