ProtParam
User-provided sequence:
10 20 30 40 50 60
MLVVQICSSP SHEMFWDISP QGKVPVLKID DKWVTDSDAT VGILEEKYPD PPLKTPAEFA
70 80 90 100 110 120
SVGSNIFEAL ENHLKSHDGP FIAGERVSAV DLSLAPKLYH LQVALGHFKS WSVPESFPHV
130 140 150
HNYMKTLFSL DSFEKTKTEE KCVISGWAPK VNP
References and
documentation are available.
Number of amino acids: 153
Molecular weight: 17061.49
Theoretical pI: 5.58
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 14
Atomic composition:
Carbon C 780
Hydrogen H 1190
Nitrogen N 194
Oxygen O 226
Sulfur S 5
Formula: C
780H
1190N
194O
226S
5
Total number of atoms: 2395
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26595
Abs 0.1% (=1 g/l) 1.559, assuming all pairs of Cys residues form cystines
Ext. coefficient 26470
Abs 0.1% (=1 g/l) 1.551, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.76
This classifies the protein as unstable.
Aliphatic index: 83.40
Grand average of hydropathicity (GRAVY): -0.204