ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MLWVDKYRPK SLDKVIVHED IAQKLKKLVS EQDCPHLLFY GPSGSGKKTL IMALLKQIYG 

        70         80         90        100        110        120 
ASAEKVKVEN RAWKVDAGSR TIDLELTTLS STNHVELTPS DAGFQDRYIV QEIIKEMAKN 

       130        140        150        160        170        180 
RPIDTKGKKG YKVLVLNEVD KLSREAQHSL RRTMEKYSSS CRLILCCNSS SKVTEAIKSR 

       190        200        210        220        230        240 
CLNVRINAPS QEEIVKVLEF VAKKESLQLP QGFAARIAEK SNRSLRRAIL SLETCRVQNY 

       250        260        270        280        290        300 
PFTGNQVISP MDWEEYVAEI ATDMMKEQSP KKLFQVRGKV YELLVNCIPP EVILKRLLHE 

       310        320        330        340        350 
LLKKLDSELK LEVCHWAAYY EHRMRLGQKA IFHIEAFVAK FMSIYKNFLI STFG 

References and documentation are available.

Number of amino acids: 354

Molecular weight: 40600.44

Theoretical pI: 9.32

Amino acid composition: 
Ala (A)  23	  6.5%
Arg (R)  20	  5.6%
Asn (N)  12	  3.4%
Asp (D)  13	  3.7%
Cys (C)   8	  2.3%
Gln (Q)  14	  4.0%
Glu (E)  29	  8.2%
Gly (G)  13	  3.7%
His (H)   8	  2.3%
Ile (I)  23	  6.5%
Leu (L)  39	 11.0%
Lys (K)  37	 10.5%
Met (M)   9	  2.5%
Phe (F)  11	  3.1%
Pro (P)  12	  3.4%
Ser (S)  28	  7.9%
Thr (T)  13	  3.7%
Trp (W)   4	  1.1%
Tyr (Y)  12	  3.4%
Val (V)  26	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 57

Atomic composition:

Carbon      C	      1818
Hydrogen    H	      2947
Nitrogen    N	       497
Oxygen      O	       518
Sulfur      S	        17

Formula: C1818H2947N497O518S17
Total number of atoms: 5797

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40380
Abs 0.1% (=1 g/l)   0.995, assuming all pairs of Cys residues form cystines


Ext. coefficient    39880
Abs 0.1% (=1 g/l)   0.982, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.11
This classifies the protein as stable.



Aliphatic index: 96.10

Grand average of hydropathicity (GRAVY): -0.290