ProtParam
User-provided sequence:
10 20 30 40 50 60
MMAARLLGTS SRIFKLNKHL HTSKVAFMPV VQFKLSDIGE GIAEVQVKEW YVKEGDTISQ
70 80 90 100 110 120
FDKVCEVQSD KAAVTISCRY DGIVKKLYHE VDGMARVGQA LIDVEIEGNV EEPEQPKKEA
130 140 150 160 170 180
ASSSPEAPKS SAPKAPESAH SEGKVLATPA VRRIAIENKI KLAEVRGTGK DGRVLKEDVL
190 200 210 220 230 240
KFLGQVPADH TSGSTNIRTT HQAPQPSSKS YEPLKEDVAV PIRGYTRAMV KTMTEALKIP
250 260 270 280 290 300
HFGYNDEINV DSLVKYRAEL KEFAKERHIK LSYMPFFIKA ASLALLEYPS LNSTTDEKME
310 320 330 340 350 360
NVIHKASHNI CLAMDTPGGL VVPNIKNCEQ RSIFEIAQEL NRLLEAGKKQ QIKREDLIDG
370 380 390 400 410 420
TFSLSNIGNI GGTYASPVVF PPQVAIGAIG KIEKLPRFDK HDNVIPVNIM KVSWCADHRV
430 440
VDGATMARFS NRWKFYLEHP SAMLAQLK
References and
documentation are available.
Number of amino acids: 448
Molecular weight: 49691.21
Theoretical pI: 8.69
Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 61
Atomic composition:
Carbon C 2210
Hydrogen H 3550
Nitrogen N 610
Oxygen O 655
Sulfur S 17
Formula: C
2210H
3550N
610O
655S
17
Total number of atoms: 7042
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33140
Abs 0.1% (=1 g/l) 0.667, assuming all pairs of Cys residues form cystines
Ext. coefficient 32890
Abs 0.1% (=1 g/l) 0.662, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.98
This classifies the protein as unstable.
Aliphatic index: 87.08
Grand average of hydropathicity (GRAVY): -0.318