ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMAARLLGTS SRIFKLNKHL HTSKVAFMPV VQFKLSDIGE GIAEVQVKEW YVKEGDTISQ 

        70         80         90        100        110        120 
FDKVCEVQSD KAAVTISCRY DGIVKKLYHE VDGMARVGQA LIDVEIEGNV EEPEQPKKEA 

       130        140        150        160        170        180 
ASSSPEAPKS SAPKAPESAH SEGKVLATPA VRRIAIENKI KLAEVRGTGK DGRVLKEDVL 

       190        200        210        220        230        240 
KFLGQVPADH TSGSTNIRTT HQAPQPSSKS YEPLKEDVAV PIRGYTRAMV KTMTEALKIP 

       250        260        270        280        290        300 
HFGYNDEINV DSLVKYRAEL KEFAKERHIK LSYMPFFIKA ASLALLEYPS LNSTTDEKME 

       310        320        330        340        350        360 
NVIHKASHNI CLAMDTPGGL VVPNIKNCEQ RSIFEIAQEL NRLLEAGKKQ QIKREDLIDG 

       370        380        390        400        410        420 
TFSLSNIGNI GGTYASPVVF PPQVAIGAIG KIEKLPRFDK HDNVIPVNIM KVSWCADHRV 

       430        440 
VDGATMARFS NRWKFYLEHP SAMLAQLK 

References and documentation are available.

Number of amino acids: 448

Molecular weight: 49691.21

Theoretical pI: 8.69

Amino acid composition: 
Ala (A)  40	  8.9%
Arg (R)  20	  4.5%
Asn (N)  17	  3.8%
Asp (D)  21	  4.7%
Cys (C)   5	  1.1%
Gln (Q)  15	  3.3%
Glu (E)  35	  7.8%
Gly (G)  26	  5.8%
His (H)  13	  2.9%
Ile (I)  31	  6.9%
Leu (L)  32	  7.1%
Lys (K)  41	  9.2%
Met (M)  12	  2.7%
Phe (F)  15	  3.3%
Pro (P)  24	  5.4%
Ser (S)  32	  7.1%
Thr (T)  19	  4.2%
Trp (W)   3	  0.7%
Tyr (Y)  11	  2.5%
Val (V)  36	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2210
Hydrogen    H	      3550
Nitrogen    N	       610
Oxygen      O	       655
Sulfur      S	        17

Formula: C2210H3550N610O655S17
Total number of atoms: 7042

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33140
Abs 0.1% (=1 g/l)   0.667, assuming all pairs of Cys residues form cystines


Ext. coefficient    32890
Abs 0.1% (=1 g/l)   0.662, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.98
This classifies the protein as unstable.



Aliphatic index: 87.08

Grand average of hydropathicity (GRAVY): -0.318