ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMARNVLVSG GAGYIGSHTV LQLLLGGYSV VVVDNLDNSS AVSLQRVKKL AAEHGERLSF 

        70         80         90        100        110        120 
HQVDLRDRSA LEKIFSETKF DAVIHFAGLK AVGESVEKPL LYYNNNLVGT ITLLEVMAQH 

       130        140        150        160        170        180 
GCKNLVFSSS ATVYGSPKEV PCTEEFPISA LNPYGRTKLF IEEICRDVYG SDPEWKIILL 

       190        200        210        220        230        240 
RYFNPVGAHP SGDIGEDPRG IPNNLMPFVQ QVAVGRRPHL TVFGNDYNTK DGTGVRDYIH 

       250        260        270        280        290        300 
VIDLADGHIA ALRKLEDCKI GCEVYNLGTG NGTSVLEMVD AFEKASGKKI PLVIAGRRPG 

       310        320        330        340        350 
DAEVVYASTE RAESELNWKA KYGIEEMCRD LWNWASNNPY GYDSSSEDNS H 

References and documentation are available.

Number of amino acids: 351

Molecular weight: 38617.69

Theoretical pI: 5.52

Amino acid composition: 
Ala (A)  25	  7.1%
Arg (R)  17	  4.8%
Asn (N)  20	  5.7%
Asp (D)  20	  5.7%
Cys (C)   6	  1.7%
Gln (Q)   6	  1.7%
Glu (E)  25	  7.1%
Gly (G)  32	  9.1%
His (H)  10	  2.8%
Ile (I)  18	  5.1%
Leu (L)  32	  9.1%
Lys (K)  18	  5.1%
Met (M)   6	  1.7%
Phe (F)  11	  3.1%
Pro (P)  15	  4.3%
Ser (S)  26	  7.4%
Thr (T)  13	  3.7%
Trp (W)   4	  1.1%
Tyr (Y)  15	  4.3%
Val (V)  32	  9.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      1715
Hydrogen    H	      2678
Nitrogen    N	       470
Oxygen      O	       522
Sulfur      S	        12

Formula: C1715H2678N470O522S12
Total number of atoms: 5397

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44725
Abs 0.1% (=1 g/l)   1.158, assuming all pairs of Cys residues form cystines


Ext. coefficient    44350
Abs 0.1% (=1 g/l)   1.148, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.18
This classifies the protein as stable.



Aliphatic index: 89.12

Grand average of hydropathicity (GRAVY): -0.222