ProtParam
User-provided sequence:
10 20 30 40 50 60
MMASLKAEET SQMQLVEREE NDEDEDLFEM IDKLIAQGIN AGDVKKLQEA GIHTCNGLMM
70 80 90 100 110 120
HTKKNLTGIK GLSEAKVDKI CEAAEKIVNF GYMTGSDALI KRKSVVKITT GCQALDDLLG
130 140 150 160 170 180
GGIETSAITE AFGEFRSGKT QLAHTLCVTT QLPTNMKGGN GKVAYIDTEG TFRPDRIVPI
190 200 210 220 230 240
AERFGMDPGA VLDNIIYARA YTYEHQYNLL LGLAAKMSEE PFRILIVDSI IALFRVDFTG
250 260 270 280 290 300
RGELADRQQK LAQMLSRLIK IAEEFNVAVY MTNQVIADPG GGMFISDPKK PAGGHVLAHA
310 320 330 340
ATIRLLFRKG KGDTRVCKVY DAPNLAEAEA SFQITQGGIA DAKD
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 37513.11
Theoretical pI: 5.53
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:
Carbon C 1649
Hydrogen H 2666
Nitrogen N 452
Oxygen O 507
Sulfur S 18
Formula: C
1649H
2666N
452O
507S
18
Total number of atoms: 5292
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 12170
Abs 0.1% (=1 g/l) 0.324, assuming all pairs of Cys residues form cystines
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.318, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 20.58
This classifies the protein as stable.
Aliphatic index: 90.26
Grand average of hydropathicity (GRAVY): -0.153