ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMDISPTCYG YIDDEQDLAL VFQGVFNGNL RCIERRPYDA EKVELVNPGN IFVFNEEKSG 

        70         80         90        100        110        120 
IKRWTDGFSW SPSRISGKFL VYREYNRLGS THDLPLHNVP EYNIFERAHR KYFYTGLLKK 

       130        140        150        160        170        180 
TFSLKFNMDP TDSTKLETFH LIAYYTEKDI HQGSLRRPSE NPFFHKFRPS QKLLDALQKV 

       190        200        210        220        230        240 
AVGNGRSNPS KNNERGRTKA HNYKTRRSLS SSPSYCDLLS NYNNHPGNIP VRTAVQLPLT 

       250        260        270        280        290        300 
TFNNAPREMH QQQHRQQQQY LLPIDEQNKL PLPYMQHQPQ PIGVYNPNYQ PGLRRTVSQP 

       310        320        330        340        350        360 
MIFCNTYNTL PQQPTAAPYE RRGVSPSVIY SSNTLSPIPY QNIDPYSSRS GPECNHSKAP 

       370        380        390        400        410        420 
IAPTMMPPVH HILVHDYRQP KPVTDSINPP NVNITTSTTN KNLDGIYILP APRMNPPAQT 

       430        440        450 
QYQMIHAPDS MQHPPTFSKN NTSSNPKSHQ YSK 

References and documentation are available.

Number of amino acids: 453

Molecular weight: 52047.65

Theoretical pI: 9.43

Amino acid composition: 
Ala (A)  16	  3.5%
Arg (R)  27	  6.0%
Asn (N)  38	  8.4%
Asp (D)  18	  4.0%
Cys (C)   5	  1.1%
Gln (Q)  28	  6.2%
Glu (E)  17	  3.8%
Gly (G)  19	  4.2%
His (H)  18	  4.0%
Ile (I)  23	  5.1%
Leu (L)  32	  7.1%
Lys (K)  23	  5.1%
Met (M)  11	  2.4%
Phe (F)  17	  3.8%
Pro (P)  48	 10.6%
Ser (S)  38	  8.4%
Thr (T)  28	  6.2%
Trp (W)   2	  0.4%
Tyr (Y)  26	  5.7%
Val (V)  19	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2313
Hydrogen    H	      3555
Nitrogen    N	       661
Oxygen      O	       682
Sulfur      S	        16

Formula: C2313H3555N661O682S16
Total number of atoms: 7227

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49990
Abs 0.1% (=1 g/l)   0.960, assuming all pairs of Cys residues form cystines


Ext. coefficient    49740
Abs 0.1% (=1 g/l)   0.956, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 64.74
This classifies the protein as unstable.



Aliphatic index: 63.05

Grand average of hydropathicity (GRAVY): -0.834