ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMDLDKIIAS LRDGKHIPEE TVFRLCLNSQ ELLMNEGNVT QVDTPVTICG DIHGQLHDLL 

        70         80         90        100        110        120 
TLFEKSGGVE KTRYIFLGDF VDRGFYSLES FLLLLCYKLR YPDRITLIRG NHETRQITKV 

       130        140        150        160        170        180 
YGFYDEVVRK YGNSNVWRYC CEVFDYLSLG AIINNSIFCV HGGLSPDMTT VDEIRTIDRK 

       190        200        210        220        230        240 
QEVPHEGAMC DLLWSDPEDV DTWSLSPRGA GFLFGKREVD QFLEKNNVEL IARAHQLVME 

       250        260        270        280        290        300 
GYKEMFDGGL VTVWSAPNYC YRCGNVAAVL KIDDDLNREY TIFEAVQAQN EVGNAIIPTK 


KSQMDYFL 

References and documentation are available.

Number of amino acids: 308

Molecular weight: 35264.19

Theoretical pI: 4.91

Amino acid composition: 
Ala (A)  12	  3.9%
Arg (R)  17	  5.5%
Asn (N)  15	  4.9%
Asp (D)  24	  7.8%
Cys (C)   9	  2.9%
Gln (Q)  10	  3.2%
Glu (E)  22	  7.1%
Gly (G)  23	  7.5%
His (H)   7	  2.3%
Ile (I)  19	  6.2%
Leu (L)  32	 10.4%
Lys (K)  14	  4.5%
Met (M)   8	  2.6%
Phe (F)  15	  4.9%
Pro (P)   9	  2.9%
Ser (S)  14	  4.5%
Thr (T)  16	  5.2%
Trp (W)   4	  1.3%
Tyr (Y)  14	  4.5%
Val (V)  24	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1575
Hydrogen    H	      2436
Nitrogen    N	       416
Oxygen      O	       470
Sulfur      S	        17

Formula: C1575H2436N416O470S17
Total number of atoms: 4914

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43360
Abs 0.1% (=1 g/l)   1.230, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   1.215, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 17.21
This classifies the protein as stable.



Aliphatic index: 91.07

Grand average of hydropathicity (GRAVY): -0.197