ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMEIRTPKTG KAKRVLESRA PKLVETGKKT LILHGTKTSA TLSSVMTELY RLKKGGAIRY 

        70         80         90        100        110        120 
SRRNENIRPF ESGGETSLEF FSQKTDCSIF VYGSHTKKRP DNLVLGRMYD HQVYDLIEVG 

       130        140        150        160        170        180 
IENFKSLRAF SYDKKFAPHE GTKPFICFIG EGFENVSELK HLKEVLTDLF RGEVVDNLNL 

       190        200        210        220        230        240 
TGLDRAYVCS AISPTKVFLT HCALKLKKSG SIVPRMELVE VGPSMDLVIR RNRLPNDSLM 

       250        260        270        280        290        300 
KEAMRTSKDK PKKKEKNVDQ DAVLGKTGKI YMPDQKLKEM KLFDKSKGSK RERKDAKLKH 

       310 
KEETVAKKMK VSSE 

References and documentation are available.

Number of amino acids: 314

Molecular weight: 35727.63

Theoretical pI: 9.74

Amino acid composition: 
Ala (A)  13	  4.1%
Arg (R)  20	  6.4%
Asn (N)  10	  3.2%
Asp (D)  16	  5.1%
Cys (C)   4	  1.3%
Gln (Q)   4	  1.3%
Glu (E)  25	  8.0%
Gly (G)  20	  6.4%
His (H)   7	  2.2%
Ile (I)  13	  4.1%
Leu (L)  30	  9.6%
Lys (K)  44	 14.0%
Met (M)  11	  3.5%
Phe (F)  13	  4.1%
Pro (P)  12	  3.8%
Ser (S)  24	  7.6%
Thr (T)  19	  6.1%
Trp (W)   0	  0.0%
Tyr (Y)   8	  2.5%
Val (V)  21	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      1581
Hydrogen    H	      2598
Nitrogen    N	       446
Oxygen      O	       462
Sulfur      S	        15

Formula: C1581H2598N446O462S15
Total number of atoms: 5102

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12170
Abs 0.1% (=1 g/l)   0.341, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.334, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.09
This classifies the protein as stable.



Aliphatic index: 76.94

Grand average of hydropathicity (GRAVY): -0.622