ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY 

        70         80         90        100        110        120 
NGRFDAKDID QIPGRVGWLT NMHATLVSQE TLSSGSNGGG NSNDGERVTT NQGISGVDFY 

       130        140        150        160        170        180 
FLDEEGGSFK STVVYDPYFF IACNDESRVN DVEELVKKYL ESCLKSLQII RKEDLTMDNH 

       190        200        210        220        230        240 
LLGLQKTLIK LSFVNSNQLF EARKLLRPIL QDNANNNVQR NIYNVAANGS EKVDAKHLIE 

       250        260        270        280        290        300 
DIREYDVPYH VRVSIDKDIR VGKWYKVTQQ GFIEDTRKIA FADPVVMAFD IETTKPPLKF 

       310        320        330        340        350        360 
PDSAVDQIMM ISYMIDGEGF LITNREIISE DIEDFEYTPK PEYPGFFTIF NENDEVALLQ 

       370        380        390        400        410        420 
RFFEHIRDVR PTVISTFNGD FFDWPFIHNR SKIHGLDMFD EIGFAPDAEG EYKSSYCSHM 

       430        440        450        460        470        480 
DCFRWVKRDS YLPQGSQGLK AVTQSKLGYN PIELDPELMT PYAFEKPQHL SEYSVSDAVA 

       490        500        510        520        530        540 
TYYLYMKYVH PFIFSLCTII PLNPDETLRK GTGTLCEMLL MVQAYQHNIL LPNKHTDPIE 

       550        560        570        580        590        600 
RFYDGHLLES ETYVGGHVES LEAGVFRSDL KNEFKIDPSA IDELLQELPE ALKFSVEVEN 

       610        620        630        640        650        660 
KSSVDKVTNF EEIKNQITQK LLELKENNIR NELPLIYHVD VASMYPNIMT TNRLQPDSIK 

       670        680        690        700        710        720 
AERDCASCDF NRPGKTCARK LKWAWRGEFF PSKMDEYNMI KRALQNETFP NKNKFSKKKV 

       730        740        750        760        770        780 
LTFDELSYAD QVIHIKKRLT EYSRKVYHRV KVSEIVEREA IVCQRENPFY VDTVKSFRDR 

       790        800        810        820        830        840 
RYEFKGLAKT WKGNLSKIDP SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR 

       850        860        870        880        890        900 
WYSMEMAGIT CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPET YFFTLENGKK 

       910        920        930        940        950        960 
LYLSYPCSML NYRVHQKFTN HQYQELKDPL NYIYETHSEN TIFFEVDGPY KAMILPSSKE 

       970        980        990       1000       1010       1020 
EGKGIKKRYA VFNEDGSLAE LKGFELKRRG ELQLIKNFQS DIFKVFLEGD TLEGCYSAVA 

      1030       1040       1050       1060       1070       1080 
SVCNRWLDVL DSHGLMLEDE DLVSLICENR SMSKTLKEYE GQKSTSITTA RRLGDFLGED 

      1090       1100       1110       1120       1130       1140 
MVKDKGLQCK YIISSKPFNA PVTERAIPVA IFSADIPIKR SFLRRWTLDP SLEDLDIRTI 

      1150       1160       1170       1180       1190       1200 
IDWGYYRERL GSAIQKIITI PAALQGVSNP VPRVEHPDWL KRKIATKEDK FKQTSLTKFF 

      1210       1220       1230       1240       1250       1260 
SKTKNVPTMG KIKDIEDLFE PTVEEDNAKI KIARTTKKKA VSKRKRNQLT NEEDPLVLPS 

      1270       1280       1290       1300       1310       1320 
EIPSMDEDYV GWLNYQKIKW KIQARDRKRR DQLFGNTNSS RERSALGSMI RKQAESYANS 

      1330       1340       1350       1360       1370       1380 
TWEVLQYKDS GEPGVLEVFV TINGKVQNIT FHIPKTIYMK FKSQTMPLQK IKNCLIEKSS 

      1390       1400       1410       1420       1430       1440 
ASLPNNPKTS NPAGGQLFKI TLPESVFLEE KENCTSIFND ENVLGVFEGT ITPHQRAIMD 

      1450       1460       1470       1480       1490       1500 
LGASVTFRSK AMGALGKGIQ QGFEMKDLSM AENERYLSGF SMDIGYLLHF PTSIGYEFFS 

      1510       1520       1530       1540       1550       1560 
LFKSWGDTIT ILVLKPSNQA QEINASSLGQ IYKQMFEKKK GKIETYSYLV DIKEDINFEF 

      1570       1580       1590       1600       1610       1620 
VYFTDISKLY RRLSQETTKL KEERGLQFLL LLQSPFITKL LGTIRLLNQM PIVKLSLNEV 

      1630       1640       1650       1660       1670       1680 
LLPQLNWQPT LLKKLVNHVL SSGSWISHLI KLSQYSNIPI CNLRLDSMDY IIDVLYARKL 

      1690       1700       1710       1720       1730       1740 
KKENIVLWWN EKAPLPDHGG IQNDFDLNTS WIMNDSEFPK INNSGVYDNV VLDVGVDNLT 

      1750       1760       1770       1780       1790       1800 
VNTILTSALI NDAEGSDLVN NNMGIDDKDA VINSPSEFVH DAFSNDALNV LRGMLKEWWD 

      1810       1820       1830       1840       1850       1860 
EALKENSTAD LLVNSLASWV QNPNAKLFDG LLRYHVHNLT KKALLQLVNE FSALGSTIVY 

      1870       1880       1890       1900       1910       1920 
ADRNQILIKT NKYSPENCYA YSQYMMKAVR TNPMFSYLDL NIKRYWDLLI WMDKFNFSGL 

      1930       1940       1950       1960       1970       1980 
ACIEIEEKEN QDYTAVSQWQ LKKFLSPIYQ PEFEDWMMII LDSMLKTKQS YLKLNSGTQR 

      1990       2000       2010       2020       2030       2040 
PTQIVNVKKQ DKEDSVENSL NGFSHLFSKP LMKRVKKLFK NQQEFILDPQ YEADYVIPVL 

      2050       2060       2070       2080       2090       2100 
PGSHLNVKNP LLELVKSLCH VMLLSKSTIL EIRTLRKELL KIFELREFAK VAEFKDPSLS 

      2110       2120       2130       2140       2150       2160 
LVVPDFLCEY CFFISDIDFC KAAPESIFSC VRCHKAFNQV LLQEHLIQKL RSDIESYLIQ 

      2170       2180       2190       2200       2210       2220 
DLRCSRCHKV KRDYMSAHCP CAGAWEGTLP RESIVQKLNV FKQVAKYYGF DILLSCIADL 


TI 

References and documentation are available.

Number of amino acids: 2222

Molecular weight: 255671.10

Theoretical pI: 6.53

Amino acid composition: 
Ala (A) 103	  4.6%
Arg (R)  97	  4.4%
Asn (N) 128	  5.8%
Asp (D) 137	  6.2%
Cys (C)  33	  1.5%
Gln (Q)  86	  3.9%
Glu (E) 155	  7.0%
Gly (G) 105	  4.7%
His (H)  40	  1.8%
Ile (I) 152	  6.8%
Leu (L) 226	 10.2%
Lys (K) 185	  8.3%
Met (M)  56	  2.5%
Phe (F) 112	  5.0%
Pro (P)  92	  4.1%
Ser (S) 165	  7.4%
Thr (T) 104	  4.7%
Trp (W)  30	  1.4%
Tyr (Y)  90	  4.1%
Val (V) 126	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 292
Total number of positively charged residues (Arg + Lys): 282

Atomic composition:

Carbon      C	     11512
Hydrogen    H	     17998
Nitrogen    N	      3022
Oxygen      O	      3380
Sulfur      S	        89

Formula: C11512H17998N3022O3380S89
Total number of atoms: 36001

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   301100
Abs 0.1% (=1 g/l)   1.178, assuming all pairs of Cys residues form cystines


Ext. coefficient   299100
Abs 0.1% (=1 g/l)   1.170, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.20
This classifies the protein as stable.



Aliphatic index: 87.43

Grand average of hydropathicity (GRAVY): -0.376