ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMHFRKKSSI SNTSDHDGAN RASDVKISED DKARLKMRTA SVADPILDAV QEAQPFEQAA 

        70         80         90        100        110        120 
DTFHDNMNRQ SYFSNEEGHV LCDVFGQPIT QADISNPTRA RDERPLDTIR SFEYAVSGDP 

       130        140        150        160        170        180 
VWAQQLETPT YGFRVRPDFP VFGAAVTYDA NGMPQQVGGA SSQMYGEQAV YQPQQHVQTE 

       190 
EKQKKKKKGL FGRMKKK 

References and documentation are available.

Number of amino acids: 197

Molecular weight: 22178.67

Theoretical pI: 6.52

Amino acid composition: 
Ala (A)  18	  9.1%
Arg (R)  12	  6.1%
Asn (N)   7	  3.6%
Asp (D)  16	  8.1%
Cys (C)   1	  0.5%
Gln (Q)  17	  8.6%
Glu (E)  11	  5.6%
Gly (G)  12	  6.1%
His (H)   5	  2.5%
Ile (I)   6	  3.0%
Leu (L)   6	  3.0%
Lys (K)  14	  7.1%
Met (M)   7	  3.6%
Phe (F)  10	  5.1%
Pro (P)  11	  5.6%
Ser (S)  14	  7.1%
Thr (T)  10	  5.1%
Trp (W)   1	  0.5%
Tyr (Y)   6	  3.0%
Val (V)  13	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	       963
Hydrogen    H	      1498
Nitrogen    N	       282
Oxygen      O	       306
Sulfur      S	         8

Formula: C963H1498N282O306S8
Total number of atoms: 3057

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.651, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.651, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.83
This classifies the protein as unstable.



Aliphatic index: 52.03

Grand average of hydropathicity (GRAVY): -0.872