ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMITRGGAKA AKSLLVAAGP RLFSTVRTVS SHEALSASHI LKPGVTSAWI WTRAPTIGGM 

        70         80         90        100        110        120 
RFASTITLGE KTPMKEEDAN QKKTENESTG GDAAGGNNKG DKGIASYWGV EPNKITKEDG 

       130        140        150        160        170        180 
SEWKWNCFRP WETYKADITI DLKKHHVPTT FLDRIAYWTV KSLRWPTDLF FQRRYGCRAM 

       190        200        210        220        230        240 
MLETVAAVPG MVGGMLLHCK SLRRFEQSGG WIKALLEEAE NERMHLMTFM EVAKPKWYER 

       250        260        270        280        290        300 
ALVITVQGVF FNAYFLGYLI SPKFAHRMVG YLEEEAIHSY TEFLKELDKG NIENVPAPAI 

       310        320        330        340        350 
AIDYWRLPAD ATLRDVVMVV RADEAHHRDV NHFASDIHYQ GRELKEAPAP IGYH 

References and documentation are available.

Number of amino acids: 354

Molecular weight: 39979.87

Theoretical pI: 8.56

Amino acid composition: 
Ala (A)  36	 10.2%
Arg (R)  21	  5.9%
Asn (N)  11	  3.1%
Asp (D)  15	  4.2%
Cys (C)   3	  0.8%
Gln (Q)   5	  1.4%
Glu (E)  27	  7.6%
Gly (G)  27	  7.6%
His (H)  13	  3.7%
Ile (I)  19	  5.4%
Leu (L)  26	  7.3%
Lys (K)  24	  6.8%
Met (M)  13	  3.7%
Phe (F)  14	  4.0%
Pro (P)  16	  4.5%
Ser (S)  17	  4.8%
Thr (T)  23	  6.5%
Trp (W)  11	  3.1%
Tyr (Y)  12	  3.4%
Val (V)  21	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1801
Hydrogen    H	      2782
Nitrogen    N	       494
Oxygen      O	       507
Sulfur      S	        16

Formula: C1801H2782N494O507S16
Total number of atoms: 5600

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78505
Abs 0.1% (=1 g/l)   1.964, assuming all pairs of Cys residues form cystines


Ext. coefficient    78380
Abs 0.1% (=1 g/l)   1.960, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.04
This classifies the protein as stable.



Aliphatic index: 76.95

Grand average of hydropathicity (GRAVY): -0.327