ProtParam
User-provided sequence:
10 20 30 40 50 60
MMITRGGAKA AKSLLVAAGP RLFSTVRTVS SHEALSASHI LKPGVTSAWI WTRAPTIGGM
70 80 90 100 110 120
RFASTITLGE KTPMKEEDAN QKKTENESTG GDAAGGNNKG DKGIASYWGV EPNKITKEDG
130 140 150 160 170 180
SEWKWNCFRP WETYKADITI DLKKHHVPTT FLDRIAYWTV KSLRWPTDLF FQRRYGCRAM
190 200 210 220 230 240
MLETVAAVPG MVGGMLLHCK SLRRFEQSGG WIKALLEEAE NERMHLMTFM EVAKPKWYER
250 260 270 280 290 300
ALVITVQGVF FNAYFLGYLI SPKFAHRMVG YLEEEAIHSY TEFLKELDKG NIENVPAPAI
310 320 330 340 350
AIDYWRLPAD ATLRDVVMVV RADEAHHRDV NHFASDIHYQ GRELKEAPAP IGYH
References and
documentation are available.
Number of amino acids: 354
Molecular weight: 39979.87
Theoretical pI: 8.56
Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1801
Hydrogen H 2782
Nitrogen N 494
Oxygen O 507
Sulfur S 16
Formula: C
1801H
2782N
494O
507S
16
Total number of atoms: 5600
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 78505
Abs 0.1% (=1 g/l) 1.964, assuming all pairs of Cys residues form cystines
Ext. coefficient 78380
Abs 0.1% (=1 g/l) 1.960, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.04
This classifies the protein as stable.
Aliphatic index: 76.95
Grand average of hydropathicity (GRAVY): -0.327