ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMLEGYTVQP PQSTLIGDIE IQDENANQEV KNVLYQGVQK GIKRLEKRQR RVALGDVTSQ 

        70         80         90        100        110        120 
KANKIHNAIH NKFHQTKNNF EIENIRSSAL VKEQQRDVRH EDSDYFLIDS SEGSSTDDEQ 

       130        140        150        160        170        180 
VNEDAIDDLL SRRVNDQQIQ ADEVYEDFDG EMQDVIEEDV DSQIEPLSPI NNDEIQTELD 

       190        200        210        220        230        240 
RAFEKYFRSV PNPLDDDTHD VVMVVEYASD IFYYLRELEV KYRPNPYYMQ NQVELTWPFR 

       250        260        270        280        290        300 
RTMIDWLVQL HFRFQLLPET LYLTINIVDR FLSKKTVTLN RFQLVGVSAL FIAAKFEEIN 

       310        320        330        340        350        360 
CPTLDDLVYM LENTYTRDDI IRAEQYMIDT LEFEIGWPGP MPFLRRISKA DDYDFEPRTL 

       370        380        390        400        410        420 
AKYLLETTIV EPKLVAAAPS WLAAGAYFLS RTILGSNDWS LKHVFYSGYT SSQIIPLASL 

       430        440        450        460 
ILENCKNASR RHHSIWKKYF DQKHYRCSQI VEEWIVSTEA 

References and documentation are available.

Number of amino acids: 460

Molecular weight: 53852.57

Theoretical pI: 4.78

Amino acid composition: 
Ala (A)  24	  5.2%
Arg (R)  27	  5.9%
Asn (N)  23	  5.0%
Asp (D)  38	  8.3%
Cys (C)   3	  0.7%
Gln (Q)  27	  5.9%
Glu (E)  39	  8.5%
Gly (G)  13	  2.8%
His (H)  10	  2.2%
Ile (I)  34	  7.4%
Leu (L)  41	  8.9%
Lys (K)  22	  4.8%
Met (M)   9	  2.0%
Phe (F)  20	  4.3%
Pro (P)  18	  3.9%
Ser (S)  29	  6.3%
Thr (T)  23	  5.0%
Trp (W)   7	  1.5%
Tyr (Y)  22	  4.8%
Val (V)  31	  6.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      2409
Hydrogen    H	      3716
Nitrogen    N	       640
Oxygen      O	       739
Sulfur      S	        12

Formula: C2409H3716N640O739S12
Total number of atoms: 7516

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    71405
Abs 0.1% (=1 g/l)   1.326, assuming all pairs of Cys residues form cystines


Ext. coefficient    71280
Abs 0.1% (=1 g/l)   1.324, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.74
This classifies the protein as unstable.



Aliphatic index: 88.35

Grand average of hydropathicity (GRAVY): -0.498