ProtParam
User-provided sequence:
10 20 30 40 50 60
MMLPRSMKFM TGRRIFHTAT VRAFQSTAKK SLTIPFLPVL PQKPGGVRGT PNDAYVPPPE
70 80 90 100 110 120
NKLEGSYHWY MEKIFALSVV PLATTAMLTT GPLSTAADSF FSVMLLGYCY MEFNSCITDY
130 140 150 160 170 180
ISERVYGVWH KYAMYMLGLG SAVSLFGIYK LETENDGVVG LVKSLWDSSE KDNSQKIEAK
K
References and
documentation are available.
Number of amino acids: 181
Molecular weight: 20248.57
Theoretical pI: 8.98
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 922
Hydrogen H 1427
Nitrogen N 229
Oxygen O 259
Sulfur S 12
Formula: C
922H
1427N
229O
259S
12
Total number of atoms: 2849
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31525
Abs 0.1% (=1 g/l) 1.557, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 1.551, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.18
This classifies the protein as unstable.
Aliphatic index: 79.17
Grand average of hydropathicity (GRAVY): -0.037