ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMMWQRYARG APRSLTSLSF GKASRISTVK PVLRSRMPVH QRLQTLSGLA TRNTIHRSTQ 

        70         80         90        100        110        120 
IRSFHISWTR LNENRPNKEG EGKNNGNKDN NSNKEDGKDK RNEFGSLSEY FRSKEFANTM 

       130        140        150        160        170        180 
FLTIGFTIIF TLLTPSSNNS GDDSNRVLTF QDFKTKYLEK GLVSKIYVVN KFLVEAELVN 

       190        200        210        220        230        240 
TKQVVSFTIG SVDIFEEQMD QIQDLLNIPP RDRIPIKYIE RSSPFTFLFP FLPTIILLGG 

       250        260        270        280        290        300 
LYFITRKINS SPPNANGGGG GGLGGMFNVG KSRAKLFNKE TDIKISFKNV AGCDEAKQEI 

       310        320        330        340        350        360 
MEFVHFLKNP GKYTKLGAKI PRGAILSGPP GTGKTLLAKA TAGEANVPFL SVSGSEFVEM 

       370        380        390        400        410        420 
FVGVGASRVR DLFTQARSMA PSIIFIDEID AIGKERGKGG ALGGANDERE ATLNQLLVEM 

       430        440        450        460        470        480 
DGFTTSDQVV VLAGTNRPDV LDNALMRPGR FDRHIQIDSP DVNGRQQIYL VHLKRLNLDP 

       490        500        510        520        530        540 
LLTDDMNNLS GKLATLTPGF TGADIANACN EAALIAARHN DPYITIHHFE QAIERVIAGL 

       550        560        570        580        590        600 
EKKTRVLSKE EKRSVAYHEA GHAVCGWFLK YADPLLKVSI IPRGQGALGY AQYLPPDQYL 

       610        620        630        640        650        660 
ISEEQFRHRM IMALGGRVSE ELHFPSVTSG AHDDFKKVTQ MANAMVTSLG MSPKIGYLSF 

       670        680        690        700        710        720 
DQNDGNFKVN KPFSNKTART IDLEVKSIVD DAHRACTELL TKNLDKVDLV AKELLRKEAI 

       730        740        750        760 
TREDMIRLLG PRPFKERNEA FEKYLDPKSN TEPPEAPAAT N 

References and documentation are available.

Number of amino acids: 761

Molecular weight: 84543.63

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  54	  7.1%
Arg (R)  47	  6.2%
Asn (N)  46	  6.0%
Asp (D)  41	  5.4%
Cys (C)   4	  0.5%
Gln (Q)  23	  3.0%
Glu (E)  46	  6.0%
Gly (G)  60	  7.9%
His (H)  15	  2.0%
Ile (I)  47	  6.2%
Leu (L)  71	  9.3%
Lys (K)  53	  7.0%
Met (M)  19	  2.5%
Phe (F)  41	  5.4%
Pro (P)  38	  5.0%
Ser (S)  50	  6.6%
Thr (T)  45	  5.9%
Trp (W)   3	  0.4%
Tyr (Y)  16	  2.1%
Val (V)  42	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 100

Atomic composition:

Carbon      C	      3756
Hydrogen    H	      5985
Nitrogen    N	      1057
Oxygen      O	      1116
Sulfur      S	        23

Formula: C3756H5985N1057O1116S23
Total number of atoms: 11937

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    40590
Abs 0.1% (=1 g/l)   0.480, assuming all pairs of Cys residues form cystines


Ext. coefficient    40340
Abs 0.1% (=1 g/l)   0.477, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.36
This classifies the protein as stable.



Aliphatic index: 83.57

Grand average of hydropathicity (GRAVY): -0.363