ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC PCGYQICQFC 

        70         80         90        100        110        120 
YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER AKLARKEKER KHREKERKEN 

       130        140        150        160        170        180 
EYTNRKHLSG TRVIQKNLVY VVGINPPVPY EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH 

       190        200        210        220        230        240 
SNNTTSEHYH HHSPGYGVYI TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR 

       250        260        270        280        290        300 
GLPCPNPNCM FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT 

       310        320        330        340        350        360 
NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS ATPVTSINLS 

       370        380        390        400        410        420 
KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS HSHGSKKKQS LAAEEYKDPY 

       430        440        450        460        470        480 
DALGNAVDFL DARLHSLSNY QKRPISIKSN IIDEETYKKY PSLFSWDKIE ASKKSDNTLA 

       490        500        510        520        530        540 
NKLVEILAIK PIDYTASVVQ FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN 

       550        560        570        580 
VSTPPPGIFG PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN 

References and documentation are available.

Number of amino acids: 587

Molecular weight: 65354.02

Theoretical pI: 8.29

Amino acid composition: 
Ala (A)  33	  5.6%
Arg (R)  24	  4.1%
Asn (N)  50	  8.5%
Asp (D)  26	  4.4%
Cys (C)  12	  2.0%
Gln (Q)  30	  5.1%
Glu (E)  32	  5.5%
Gly (G)  31	  5.3%
His (H)  21	  3.6%
Ile (I)  34	  5.8%
Leu (L)  39	  6.6%
Lys (K)  38	  6.5%
Met (M)   8	  1.4%
Phe (F)  15	  2.6%
Pro (P)  41	  7.0%
Ser (S)  50	  8.5%
Thr (T)  51	  8.7%
Trp (W)   2	  0.3%
Tyr (Y)  23	  3.9%
Val (V)  27	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2845
Hydrogen    H	      4479
Nitrogen    N	       821
Oxygen      O	       908
Sulfur      S	        20

Formula: C2845H4479N821O908S20
Total number of atoms: 9073

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46020
Abs 0.1% (=1 g/l)   0.704, assuming all pairs of Cys residues form cystines


Ext. coefficient    45270
Abs 0.1% (=1 g/l)   0.693, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.81
This classifies the protein as unstable.



Aliphatic index: 67.46

Grand average of hydropathicity (GRAVY): -0.734