ProtParam
User-provided sequence:
10 20 30 40 50 60
MMPTYLGKLT WSYFFTTLGL ACAYNVTEQM EFDQFKSDYL ACLAPEHRNI VVDLASNGFI
70 80 90 100 110 120
TISPMANATI DFEDVTSDYF NCTDVNTNVQ VTIASFYNEY GFGPDDNGYY HAMEEPSEFE
130 140 150 160 170 180
RHDMDVRLYR PYYPGEFVMG RRSDALGVTG FDQKDCAGEG FYDEQTAATS CQNIGSTQYA
190 200 210 220 230
KSVRSYNYGC CGGAVWIRIW PHHNCSKGHD HHFKIRPGQM LCWNVNPYSW MQPESGWNP
References and
documentation are available.
Number of amino acids: 239
Molecular weight: 27312.33
Theoretical pI: 4.80
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 15
Atomic composition:
Carbon C 1216
Hydrogen H 1758
Nitrogen N 318
Oxygen O 369
Sulfur S 18
Formula: C
1216H
1758N
318O
369S
18
Total number of atoms: 3679
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 58830
Abs 0.1% (=1 g/l) 2.154, assuming all pairs of Cys residues form cystines
Ext. coefficient 58330
Abs 0.1% (=1 g/l) 2.136, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.51
This classifies the protein as unstable.
Aliphatic index: 53.05
Grand average of hydropathicity (GRAVY): -0.472