ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMSDEDNTYS DDEEWLDLDL GLPNNDIKTA PILNSSLLSS AVHGERLIQE RPTHDFGDVE 

        70         80         90        100        110        120 
ATVDRTVEKR SRLKITSVDR KIRLQIHQLH LLCLTYHLCT RNTWCDDNRL NYLVKYIPPG 

       130        140        150        160        170        180 
IRVSLHPSSQ KSQMIRNKTF LHGLAGLVEV WKRKYKITTN GLRKPNYGLL QNNSLISESL 

       190        200        210        220        230        240 
SLEEFCKNSK LLSGSRDYGT QLFASILRNL NVPTRLVFSL QVLSFRFKGA INEASSHEIV 

       250        260        270        280        290        300 
PAWSQQMEND SSSDISESAH ITSRFRKRRK IIQPSFSNLS HLDASDIVTE DTKLKVIDSP 

       310        320        330        340        350        360 
KPVFWVEAFN KAMQKWVCVD PFGDASVIGK YRRFEPASSD HLNQMTYVFA IEANGYVKDV 

       370        380        390        400        410        420 
TRKYCLHYYK ILKNRVEIFP FGKAWMNRIF SKIGKPRDFY NDMDAIEDAE LLRLEQSEGI 

       430        440        450        460        470        480 
PRNIQDLKDH PLFVLERHLK KNQAIKTGKS CGRINTKNGV ELVYPRKYVS NGFSAEHWYR 

       490        500        510        520        530        540 
KGRIIKPGAQ PLKHVKNGDK VLPLYDEEAT QLYTPKPVVA NIVPKNAYGN IDLYVPSMLP 

       550        560        570        580        590        600 
YGAYHCRKRC ALAAAKFLEI DYAKAVVGFD FQRKYSKPKL EGVVVSKRYE EAIDLIAEEI 

       610        620        630 
DQEEKEAEAR NVRKTCLLLW KRLITGLRIR QRVFEEYG 

References and documentation are available.

Number of amino acids: 638

Molecular weight: 73519.35

Theoretical pI: 9.23

Amino acid composition: 
Ala (A)  36	  5.6%
Arg (R)  44	  6.9%
Asn (N)  34	  5.3%
Asp (D)  37	  5.8%
Cys (C)  10	  1.6%
Gln (Q)  21	  3.3%
Glu (E)  41	  6.4%
Gly (G)  30	  4.7%
His (H)  17	  2.7%
Ile (I)  41	  6.4%
Leu (L)  64	 10.0%
Lys (K)  53	  8.3%
Met (M)   9	  1.4%
Phe (F)  24	  3.8%
Pro (P)  28	  4.4%
Ser (S)  47	  7.4%
Thr (T)  25	  3.9%
Trp (W)   9	  1.4%
Tyr (Y)  27	  4.2%
Val (V)  41	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      3295
Hydrogen    H	      5209
Nitrogen    N	       921
Oxygen      O	       949
Sulfur      S	        19

Formula: C3295H5209N921O949S19
Total number of atoms: 10393

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    90355
Abs 0.1% (=1 g/l)   1.229, assuming all pairs of Cys residues form cystines


Ext. coefficient    89730
Abs 0.1% (=1 g/l)   1.220, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.87
This classifies the protein as stable.



Aliphatic index: 88.46

Grand average of hydropathicity (GRAVY): -0.479