ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMSLINLDLV ISAVTSIANP VIKEKILRSE TVIKLLQQFN LDPEHPPADF SGVYAYTLVE 

        70         80         90        100        110        120 
YGVGKPKAFL ELFRQEAIKQ AFRKALDHNN PSILLSEVDT FLDACTLGDE IRSLELDVRR 

       130        140        150        160        170        180 
EVAAFATVFI EVAKRSRTPA DVLMNQQIGS LHKRIAGIQE QLERLPTLEG IRTEIARLAA 

       190        200        210        220        230        240 
QNYPALTPTA TENQCRAIAL AQQMRGWFET LGYRLEKYEI WAEEYFEWII NVPVRRSYDR 

       250        260        270        280        290        300 
ILVRGVAGEV RLSDVMALCQ SVNQQKTDEG WLVSTRRISR AARDEVKKEE NRHLDCFTFD 

       310        320        330        340        350        360 
ELIDLDADFS GYLDWLEAEI KRRKIDQKYV PLACTKEEID PVTKRRIGIS RYEAEDGWID 

       370        380        390        400        410        420 
GYIDLWLDDP AKEHISILGE FGTGKTWFVF HYAWTALQRY KDAQRRGVER PRLPLVITLR 

       430        440        450        460        470        480 
DFAKALNVEN VLAGFFFTQH NIRLNSEVFD QLNRMGKLLL IFDGFDEMAA KVDRQQMINN 

       490        500        510        520        530        540 
FWELAKVVVP GSKVILTCRT EHFPEAKEGR ALLNAELQAS TNKLTGETPQ FEVLELEKFN 

       550        560        570        580        590        600 
DEQIRQVLLY QAEEATVEQI MGNSQLLDLA RRPVMTDLIL EALPDIEAGK PIDMSRVYLY 

       610        620        630        640        650        660 
AVRHKMERDI KAERTFNSLA DKLYFLCELS WEMLSTDQMS LNYRLFPERI RRLFGSVVQE 

       670        680        690        700        710        720 
EKDLDHWHYD MMAQTMLVRN ADGDYTPAHR SLLEFFVAYK FAAELGALAR DFTEVGQAQS 

       730        740        750        760        770        780 
GLDSSTASID YTWSGYFSRQ LDSTGCREVI APLRQFKSEF LDKLRETFGK AKLTKAVIDL 

       790        800        810        820        830        840 
LLPMLDDNEP LIKIIEATRG RTEDEVNYVG GNAATLLVKL NKMVLEGRDL SHTVIIGADF 

       850        860        870        880        890        900 
TNTSLRCVNF TEANLAYSVF TKILGSVLTV AFSPDGKLFA TGDSGGIVRF WEAATGKELL 

       910        920        930        940        950        960 
TCKGHNSWVN SVGFSQDGKM LASGSDDQTV RLWDISSGQC LKTFKGHTSR VRSVVFSPNS 

       970        980        990       1000       1010       1020 
LMLASGSSDQ TVRLWDISSG ECLYIFQGHT GWVYSVAFNL DGSMLATGSG DQTVRLWDIS 

      1030       1040       1050       1060       1070       1080 
SSQCFYIFQG HTSCVRSVVF SSDGAMLASG SDDQTVRLWD ISSGNCLYTL QGHTSCVRSV 

      1090       1100       1110       1120       1130       1140 
VFSPDGAMLA SGGDDQIVRL WDISSGNCLY TLQGYTSWVR FLVFSPNGVT LANGSSDQIV 

      1150       1160       1170       1180       1190       1200 
RLWDISSKKC LYTLQGHTNW VNAVAFSPDG ATLASGSGDQ TVRLWDISSS KCLYILQGHT 

      1210       1220       1230       1240       1250       1260 
SWVNSVVFNP DGSTLASGSS DQTVRLWEIN SSKCLCTFQG HTSWVNSVVF NPDGSMLASG 

      1270       1280       1290       1300       1310       1320 
SSDKTVRLWD ISSSKCLHTF QGHTNWVNSV AFNPDGSMLA SGSGDQTVRL WEISSSKCLH 

      1330       1340       1350       1360       1370       1380 
TFQGHTSWVS SVTFSPDGTM LASGSDDQTV RLWSISSGEC LYTFLGHTNW VGSVIFSPDG 

      1390       1400       1410       1420       1430       1440 
AILASGSGDQ TVRLWSISSG KCLYTLQGHN NWVGSIVFSP DGTLLASGSD DQTVRLWNIS 

      1450       1460       1470       1480       1490       1500 
SGECLYTLHG HINSVRSVAF SSDGLILASG SDDETIKLWD VKTGECIKTL KSEKIYEGMN 

      1510       1520 
ITSVRGLTEV EKATLKTLGA VENREI 

References and documentation are available.

Number of amino acids: 1526

Molecular weight: 170376.98

Theoretical pI: 5.35

Amino acid composition: 
Ala (A) 102	  6.7%
Arg (R)  85	  5.6%
Asn (N)  57	  3.7%
Asp (D)  99	  6.5%
Cys (C)  27	  1.8%
Gln (Q)  61	  4.0%
Glu (E)  89	  5.8%
Gly (G) 108	  7.1%
His (H)  29	  1.9%
Ile (I)  81	  5.3%
Leu (L) 161	 10.6%
Lys (K)  65	  4.3%
Met (M)  28	  1.8%
Phe (F)  68	  4.5%
Pro (P)  38	  2.5%
Ser (S) 145	  9.5%
Thr (T)  98	  6.4%
Trp (W)  38	  2.5%
Tyr (Y)  38	  2.5%
Val (V) 109	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 188
Total number of positively charged residues (Arg + Lys): 150

Atomic composition:

Carbon      C	      7577
Hydrogen    H	     11828
Nitrogen    N	      2060
Oxygen      O	      2302
Sulfur      S	        55

Formula: C7577H11828N2060O2302S55
Total number of atoms: 23822

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   267245
Abs 0.1% (=1 g/l)   1.569, assuming all pairs of Cys residues form cystines


Ext. coefficient   265620
Abs 0.1% (=1 g/l)   1.559, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.45
This classifies the protein as unstable.



Aliphatic index: 89.25

Grand average of hydropathicity (GRAVY): -0.159