ProtParam
User-provided sequence:
10 20 30 40 50 60
MMTNSDSDHC TPAKTSISGD GLEKESDFKK QLNSEIDPQA SSSQNDAITM EDGAVSVNNR
70 80 90 100 110 120
DLQEIKESSF SKGSEQYRVD GALEESLNFE EKEQESEAQR LLEAEKRRLL AEIELGSIFR
130 140 150 160 170 180
KSVDVDTLPK IEETMDNDVD KIELVDHTAL VDVVHHPKRP GTAQNEKDTP RKLKKIGDKR
190 200 210 220 230 240
NVIEGSGVEN NGKQFRRLYT RKQLESMRFA HIVNQKNLWS EMYSRILPEV VTEYESLVYV
250 260 270 280 290 300
KNYKSSKSNR VRGRTESGNE ENLGTEEGTE DPEDYTDDND DYNSILRPAF EVDGEPDFST
310 320 330 340 350 360
GPPEDGLEYL RRVRWEAKGI PNVRVAKIDE STYIKKEQSV YMPLIPEIPK CPEYLLPMKE
370 380 390 400 410 420
WEDSLLLDFV HLRQTLTQSA NSCEDEIISS QCVEDLLVEM FNKHLHTEED ESFGEVVTDI
430 440 450 460 470 480
QGMDSVTRVS KLKKRICLVE KESGLQSSDC KWVVALCASL ETPLDADTCA CLRGLLRKCA
490 500 510
SVRAETSLEV GDEEVITMAN MLITIAGRYF GQMGQ
References and
documentation are available.
Number of amino acids: 515
Molecular weight: 58339.21
Theoretical pI: 4.72
Total number of negatively charged residues (Asp + Glu): 101
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 2516
Hydrogen H 4026
Nitrogen N 700
Oxygen O 845
Sulfur S 23
Formula: C
2516H
4026N
700O
845S
23
Total number of atoms: 8110
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41995
Abs 0.1% (=1 g/l) 0.720, assuming all pairs of Cys residues form cystines
Ext. coefficient 41370
Abs 0.1% (=1 g/l) 0.709, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.47
This classifies the protein as unstable.
Aliphatic index: 77.75
Grand average of hydropathicity (GRAVY): -0.688