ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MMTTTTNTMA MLQNLVFSVP ISRMVVRRHS LATTFSAAAT TVVPSPKPVS AKPARTPHVD 

        70         80         90        100        110        120 
SHVLIGMSEP ELQELAINLG QEGYRGKQLH HLIYKRKVNK VEDFSNLPLT FRKGLVDGGF 

       130        140        150        160        170        180 
KVGRSPIYQT VTATDGTIKL LLKLEDNLLI ETVGIPVQDD EKGITRLTAC VSSQVGCPLR 

       190        200        210        220        230        240 
CSFCATGKGG FSRNLQRHEI IEQVLAIEDV FKHRVTNVVF MGMGEPMLNL KSVLDAHRCL 

       250        260        270        280        290        300 
NKDIEIGQRM ITISTVGVPN TIKKLASHKL QSTLAVSLHA PNQSLREKIV PSAKAYPLEA 

       310        320        330        340        350        360 
IMKDCRDYFQ ETNRRVSFEY ALLAGVNDQV EHAVELAELL REWGKTYHVN LIPYNPIEGS 

       370        380        390        400        410        420 
EYQRPYKKAV LAFAAALESR KITASVRQTR GLDASAACGQ LRNKFQKSPL LTETDSQESQ 


PDAEAVAC 

References and documentation are available.

Number of amino acids: 428

Molecular weight: 47350.68

Theoretical pI: 9.05

Amino acid composition: 
Ala (A)  35	  8.2%
Arg (R)  25	  5.8%
Asn (N)  17	  4.0%
Asp (D)  16	  3.7%
Cys (C)   8	  1.9%
Gln (Q)  20	  4.7%
Glu (E)  27	  6.3%
Gly (G)  24	  5.6%
His (H)  12	  2.8%
Ile (I)  22	  5.1%
Leu (L)  44	 10.3%
Lys (K)  27	  6.3%
Met (M)  11	  2.6%
Phe (F)  13	  3.0%
Pro (P)  21	  4.9%
Ser (S)  29	  6.8%
Thr (T)  30	  7.0%
Trp (W)   1	  0.2%
Tyr (Y)  10	  2.3%
Val (V)  36	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2089
Hydrogen    H	      3396
Nitrogen    N	       592
Oxygen      O	       621
Sulfur      S	        19

Formula: C2089H3396N592O621S19
Total number of atoms: 6717

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20900
Abs 0.1% (=1 g/l)   0.441, assuming all pairs of Cys residues form cystines


Ext. coefficient    20400
Abs 0.1% (=1 g/l)   0.431, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.19
This classifies the protein as unstable.



Aliphatic index: 92.71

Grand average of hydropathicity (GRAVY): -0.186