ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNAAASSYPM ASLYVGDLHS DVTEAMLYEK FSPAGPVLSI RVCRDMITRR SLGYAYVNFQ 

        70         80         90        100        110        120 
QPADAERALD TMNFDVIKGK PIRIMWSQRD PSLRKSGVGN VFIKNLDKSI DNKALYDTFS 

       130        140        150        160        170        180 
AFGNILSCKV VCDENGSKGY AFVHFETQEA ADKAIEKMNG MLLNDRKVFV GRFKSRKERE 

       190        200        210        220        230        240 
AELGAKAKEF TNVYIKNFGE EVDDESLKEL FSQFGKTLSV KVMRDPNGKS KGFGFVSYEK 

       250        260        270        280        290        300 
HEDANKAVEE MNGKEISGKI IFVGRAQKKV ERQAELKRKF EQLKQERISR YQGVNLYIKN 

       310        320        330        340        350        360 
LDDTIDDEKL RKEFSPFGSI TSAKVMLEDG RSKGFGFVCF SSPEEATKAV TEMNGRIVGS 

       370        380        390        400        410        420 
KPLYVALAQR KEERKAHLTN QYMQRVAGMR ALPANAILNQ FQPAAGGYFV PAVPQAQGRP 

       430        440        450        460        470        480 
PYYTPNQLAQ MRPNPRWQQG GRPQGFQGMP SAIRQSGPRP TLRHLAPTGS ECPDRLAMDF 

       490        500        510        520        530        540 
GGAGAAQQGL TDSCQSGGVP TAVQNLAPRA AVAAAAPRAV APYKYASSVR SPHPAIQPLQ 

       550        560        570        580        590        600 
APQPAVHVQG QEPLTASMLA AAPPQEQKQM LGERLFPLIQ TMHSNLAGKI TGMLLEIDNS 

       610        620        630        640 
ELLHMLESPE SLRSKVDEAV AVLQAHHAKK EAAQKVGAVA AATS 

References and documentation are available.

Number of amino acids: 644

Molecular weight: 70782.85

Theoretical pI: 9.31

Amino acid composition: 
Ala (A)  73	 11.3%
Arg (R)  38	  5.9%
Asn (N)  26	  4.0%
Asp (D)  27	  4.2%
Cys (C)   6	  0.9%
Gln (Q)  39	  6.1%
Glu (E)  42	  6.5%
Gly (G)  48	  7.5%
His (H)  11	  1.7%
Ile (I)  24	  3.7%
Leu (L)  47	  7.3%
Lys (K)  47	  7.3%
Met (M)  22	  3.4%
Phe (F)  28	  4.3%
Pro (P)  39	  6.1%
Ser (S)  44	  6.8%
Thr (T)  21	  3.3%
Trp (W)   2	  0.3%
Tyr (Y)  18	  2.8%
Val (V)  42	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      3119
Hydrogen    H	      4966
Nitrogen    N	       894
Oxygen      O	       931
Sulfur      S	        28

Formula: C3119H4966N894O931S28
Total number of atoms: 9938

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    38195
Abs 0.1% (=1 g/l)   0.540, assuming all pairs of Cys residues form cystines


Ext. coefficient    37820
Abs 0.1% (=1 g/l)   0.534, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.02
This classifies the protein as unstable.



Aliphatic index: 73.25

Grand average of hydropathicity (GRAVY): -0.443