ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNALKYFSNH LITTKKQKKI NVEVTKNQDL LGPSKEVSNK YTSHSENDCV SEVDQQYDHS 

        70         80         90        100        110        120 
SSHLKESDQN QERKNSVPKK PKALRSILIE KIASILWALL LFLPYYLIIK PLMSLWFVFT 

       130        140        150        160        170        180 
FPLSVIERRV KHTDKRNRGS NASENELPVS SSNINDSSEK TNPKNCNLNT IPEAVEDDLN 

       190        200        210        220        230        240 
ASDEIILQRD NVKGSLLRAQ SVKSRPRSYS KSELSLSNHS SSNTVFGTKR MGRFLFPKKL 

       250        260        270        280        290        300 
IPKSVLNTQK KKKLVIDLDE TLIHSASRST THSNSSQGHL VEVKFGLSGI RTLYFIHKRP 

       310        320        330        340        350        360 
YCDLFLTKVS KWYDLIIFTA SMKEYADPVI DWLESSFPSS FSKRYYRSDC VLRDGVGYIK 

       370        380        390        400        410        420 
DLSIVKDSEE NGKGSSSSLD DVIIIDNSPV SYAMNVDNAI QVEGWISDPT DTDLLNLLPF 

       430        440 
LEAMRYSTDV RNILALKHGE KAFNIN 

References and documentation are available.

Number of amino acids: 446

Molecular weight: 50641.56

Theoretical pI: 8.99

Amino acid composition: 
Ala (A)  16	  3.6%
Arg (R)  20	  4.5%
Asn (N)  31	  7.0%
Asp (D)  29	  6.5%
Cys (C)   4	  0.9%
Gln (Q)  11	  2.5%
Glu (E)  24	  5.4%
Gly (G)  14	  3.1%
His (H)  11	  2.5%
Ile (I)  30	  6.7%
Leu (L)  47	 10.5%
Lys (K)  41	  9.2%
Met (M)   6	  1.3%
Phe (F)  16	  3.6%
Pro (P)  18	  4.0%
Ser (S)  60	 13.5%
Thr (T)  20	  4.5%
Trp (W)   5	  1.1%
Tyr (Y)  15	  3.4%
Val (V)  28	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2251
Hydrogen    H	      3588
Nitrogen    N	       616
Oxygen      O	       690
Sulfur      S	        10

Formula: C2251H3588N616O690S10
Total number of atoms: 7155

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50100
Abs 0.1% (=1 g/l)   0.989, assuming all pairs of Cys residues form cystines


Ext. coefficient    49850
Abs 0.1% (=1 g/l)   0.984, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.75
This classifies the protein as unstable.



Aliphatic index: 89.13

Grand average of hydropathicity (GRAVY): -0.475