ProtParam
User-provided sequence:
10 20 30 40 50 60
MNATHCILAL QLFLMAVSGC YCHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD
70 80 90 100 110 120
GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE
130 140 150
VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC
References and
documentation are available.
Number of amino acids: 155
Molecular weight: 17906.66
Theoretical pI: 8.72
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 18
Atomic composition:
Carbon C 799
Hydrogen H 1266
Nitrogen N 220
Oxygen O 231
Sulfur S 8
Formula: C
799H
1266N
220O
231S
8
Total number of atoms: 2524
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 15720
Abs 0.1% (=1 g/l) 0.878, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 0.864, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.13
This classifies the protein as unstable.
Aliphatic index: 97.48
Grand average of hydropathicity (GRAVY): -0.128