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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNATHCILAL QLFLMAVSGC YCHGTVIESL ESLNNYFNSS GIDVEEKSLF LDIWRNWQKD

70 80 90 100 110 120
GDMKILQSQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT TFFSNSKAKK DAFMSIAKFE

130 140 150
VNNPQVQRQA FNELIRVVHQ LLPESSLRKR KRSRC


References and documentation are available.

Number of amino acids: 155 Molecular weight: 17906.66 Theoretical pI: 8.72
Amino acid composition: 
Ala (A) 8 5.2% Arg (R) 8 5.2% Asn (N) 12 7.7% Asp (D) 6 3.9% Cys (C) 4 2.6% Gln (Q) 9 5.8% Glu (E) 9 5.8% Gly (G) 4 2.6% His (H) 4 2.6% Ile (I) 13 8.4% Leu (L) 17 11.0% Lys (K) 10 6.5% Met (M) 4 2.6% Phe (F) 10 6.5% Pro (P) 2 1.3% Ser (S) 17 11.0% Thr (T) 4 2.6% Trp (W) 2 1.3% Tyr (Y) 3 1.9% Val (V) 9 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 15 Total number of positively charged residues (Arg + Lys): 18 Atomic composition: Carbon C 799 Hydrogen H 1266 Nitrogen N 220 Oxygen O 231 Sulfur S 8 Formula: C799H1266N220O231S8 Total number of atoms: 2524 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 15720 Abs 0.1% (=1 g/l) 0.878, assuming all pairs of Cys residues form cystines Ext. coefficient 15470 Abs 0.1% (=1 g/l) 0.864, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 59.13 This classifies the protein as unstable. Aliphatic index: 97.48 Grand average of hydropathicity (GRAVY): -0.128