ProtParam
User-provided sequence:
10 20 30 40 50 60
MNCKNMDTSY EIINYLTKDE LDIDLSCMDK KERYKIWKRL PKCELHCHLD VCFSVDFFLN
70 80 90 100 110 120
VIRKYNIQPN MSDEEIIDYY LFSKPGKSLD EFVEKALRLT DIYIDYTVVE DLAKHAVFNK
130 140 150 160 170 180
YKEGVVLMEF RYSPSFMSFK HNLDKDLIHE AIVKGLNEAV ALLEYKIQVG LLCTGDGGLS
190 200 210 220 230 240
HERMKEAAEF CIKHKKDFVG YDHAGHEVDL KPFKDIFDNI REEGISLSVH AGEDVSIPNL
250 260 270 280 290 300
NSLYTAINLL HVKRIGHGIR VSESQELIDL VKEKDILLEV CPISNVLLNN VKSMDTHPIR
310 320 330 340 350 360
MLYDAGVKVS VNSDDPGMFL TNITDNYEEL YTHLNFTLAD FMKMNLWAVQ KSFVDPDIKN
KIISKYF
References and
documentation are available.
Number of amino acids: 367
Molecular weight: 42465.91
Theoretical pI: 5.41
Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1915
Hydrogen H 2987
Nitrogen N 487
Oxygen O 562
Sulfur S 20
Formula: C
1915H
2987N
487O
562S
20
Total number of atoms: 5971
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36830
Abs 0.1% (=1 g/l) 0.867, assuming all pairs of Cys residues form cystines
Ext. coefficient 36330
Abs 0.1% (=1 g/l) 0.856, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.21
This classifies the protein as stable.
Aliphatic index: 97.41
Grand average of hydropathicity (GRAVY): -0.220