ProtParam
User-provided sequence:
10 20 30 40 50 60
MNCLMFFKML LAFSFISLFY VGNAQQSYGN STVSALFAFG DSILDTGNNN LLLSVSKVNF
70 80 90 100 110 120
YPYGRDFIGG RATGRFGNGR VFSDIIAEGL GLKNLLPAYR DPYLWNNDLT TGVCFASGGS
130 140 150 160 170 180
GLDPITARTT GSIWVSDQVT DFQNYITRLN GVVGNQEQAN AVISNAVYLI SAGNNDIAIT
190 200 210 220 230 240
YFTTGARRLQ YTLPAYNDQL VSWTRDLIKS LYDMGARKFA VMGTLPLGCL PGARALTRAC
250 260 270 280 290 300
ELFVNQGAAM FNQQLSADID NLGATFPGAK FVYVDMYNPL LGLIINPQAS GFIDVADACC
310 320 330 340
CTPTHLIPCL DASRYVFWDV AHPTQKSYET IAPQIIENIK AKLA
References and
documentation are available.
Number of amino acids: 344
Molecular weight: 37502.82
Theoretical pI: 5.69
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 23
Atomic composition:
Carbon C 1692
Hydrogen H 2600
Nitrogen N 442
Oxygen O 493
Sulfur S 15
Formula: C
1692H
2600N
442O
493S
15
Total number of atoms: 5242
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46340
Abs 0.1% (=1 g/l) 1.236, assuming all pairs of Cys residues form cystines
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 1.222, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 22.85
This classifies the protein as stable.
Aliphatic index: 92.50
Grand average of hydropathicity (GRAVY): 0.156