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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNCLMFFKML LAFSFISLFY VGNAQQSYGN STVSALFAFG DSILDTGNNN LLLSVSKVNF

70 80 90 100 110 120
YPYGRDFIGG RATGRFGNGR VFSDIIAEGL GLKNLLPAYR DPYLWNNDLT TGVCFASGGS

130 140 150 160 170 180
GLDPITARTT GSIWVSDQVT DFQNYITRLN GVVGNQEQAN AVISNAVYLI SAGNNDIAIT

190 200 210 220 230 240
YFTTGARRLQ YTLPAYNDQL VSWTRDLIKS LYDMGARKFA VMGTLPLGCL PGARALTRAC

250 260 270 280 290 300
ELFVNQGAAM FNQQLSADID NLGATFPGAK FVYVDMYNPL LGLIINPQAS GFIDVADACC

310 320 330 340
CTPTHLIPCL DASRYVFWDV AHPTQKSYET IAPQIIENIK AKLA


References and documentation are available.

Number of amino acids: 344 Molecular weight: 37502.82 Theoretical pI: 5.69
Amino acid composition: 
Ala (A) 34 9.9% Arg (R) 14 4.1% Asn (N) 25 7.3% Asp (D) 20 5.8% Cys (C) 8 2.3% Gln (Q) 14 4.1% Glu (E) 5 1.5% Gly (G) 30 8.7% His (H) 2 0.6% Ile (I) 22 6.4% Leu (L) 36 10.5% Lys (K) 9 2.6% Met (M) 7 2.0% Phe (F) 21 6.1% Pro (P) 14 4.1% Ser (S) 21 6.1% Thr (T) 22 6.4% Trp (W) 4 1.2% Tyr (Y) 16 4.7% Val (V) 20 5.8% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 25 Total number of positively charged residues (Arg + Lys): 23 Atomic composition: Carbon C 1692 Hydrogen H 2600 Nitrogen N 442 Oxygen O 493 Sulfur S 15 Formula: C1692H2600N442O493S15 Total number of atoms: 5242 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 46340 Abs 0.1% (=1 g/l) 1.236, assuming all pairs of Cys residues form cystines Ext. coefficient 45840 Abs 0.1% (=1 g/l) 1.222, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 22.85 This classifies the protein as stable. Aliphatic index: 92.50 Grand average of hydropathicity (GRAVY): 0.156