ProtParam
User-provided sequence:
10 20 30 40 50 60
MNCTRVLQIL NPRPMPSPIM PVDVAMRICL AHSPPLRSFL SSYEDCKSRN LVNQYKPLRS
70 80 90 100 110 120
CISSKTEDDT ANITWKSPET KAKKKVVFAD SKGMSLTAVH VFKEFEEDLM LDLQFELSDL
130 140 150 160 170 180
EDAIVGLKAE KEKNFSLGFP QPAADYLDFR NRLKKNLVCL ENCIIQERSV TGTVKVSNVS
190 200 210 220 230 240
FEKVVHVRIT FDSWKSHTDI PCTYMNNVYG CEDVDTFSFS IDLPSFVAPH EQVEFCLSYK
250 260 270 280 290 300
THDMTYWDNN DGKNYKLVHT ENDSSQASVI QNATTDFKAQ GKRPDMEFDQ FGSPRTSSGF
310
FPEWQSWGHI ENNTPYW
References and
documentation are available.
Number of amino acids: 317
Molecular weight: 36323.05
Theoretical pI: 5.55
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 1614
Hydrogen H 2474
Nitrogen N 428
Oxygen O 492
Sulfur S 18
Formula: C
1614H
2474N
428O
492S
18
Total number of atoms: 5026
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46910
Abs 0.1% (=1 g/l) 1.291, assuming all pairs of Cys residues form cystines
Ext. coefficient 46410
Abs 0.1% (=1 g/l) 1.278, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.71
This classifies the protein as unstable.
Aliphatic index: 68.52
Grand average of hydropathicity (GRAVY): -0.490