ProtParam
User-provided sequence:
10 20 30 40 50 60
MNDDSQDKII HDIRIQLRKA ATELSRWKLY GSSKWAAEAL AGLAEAIDVD QTHSLADESP
70 80 90 100 110 120
LRNKQGVPKQ MFEIPQNGFG LSETEYDLYL LGSTLFDAKE FDRCVFFLKD VTNPYLKFLK
130 140 150 160 170 180
LYSKFLSWDK KSQESMENIL TTGKFTDEMY RANKDGDGSG NEDINQSGHQ RANLKMVSNE
190 200 210 220 230 240
HESQSNISSI LKEINTFLES YEIKIDDDEA DLGLALLYYL RGVILKQEKN ISKAMSSFLK
250 260 270 280 290 300
SLSCYSFNWS CWLELMDCLQ KVDDALLLNN YLYQNFQFKF SENLGSQRTI EFNIMIKFFK
310 320 330 340 350 360
LKVFEELNGQ LEDYFEDLEF LLQVFPNFTF LKAYNATISY NNLDYVTAES RFDDIVKQDP
370 380 390 400 410 420
YRLNDLETYS NILYVMQKNS KLAYLAQFVS QIDRFRPETC CIIANYYSAR QEHEKSIMYF
430 440 450 460 470 480
RRALTLDKKT TNAWTLMGHE FVELSNSHAA IECYRRAVDI CPRDFKAWFG LGQAYALLDM
490 500 510 520 530 540
HLYSLYYFQK ACTLKPWDRR IWQVLGECYS KTGNKVEAIK CYKRSIKASQ TVDQNTSIYY
550 560 570 580 590 600
RLAQLYEELE DLQECKKFMM KCVDVEELLE GIVTDETVKA RLWLAIFEIK AGNYQLAYDY
610 620
AMGVSSGTSQ EIEEARMLAR ECRRHM
References and
documentation are available.
Number of amino acids: 626
Molecular weight: 73114.17
Theoretical pI: 5.29
Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 75
Atomic composition:
Carbon C 3284
Hydrogen H 5049
Nitrogen N 849
Oxygen O 981
Sulfur S 31
Formula: C
3284H
5049N
849O
981S
31
Total number of atoms: 10194
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 109515
Abs 0.1% (=1 g/l) 1.498, assuming all pairs of Cys residues form cystines
Ext. coefficient 108640
Abs 0.1% (=1 g/l) 1.486, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.60
This classifies the protein as unstable.
Aliphatic index: 83.40
Grand average of hydropathicity (GRAVY): -0.405