ProtParam
User-provided sequence:
10 20 30 40 50 60
MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI
70 80 90 100 110 120
CVRCATQRNI CQCCMLDSRW HIPIQLRDHL ISLVNEENVM TEEAKNDMMK RFLSLKNVKL
130 140 150 160 170 180
GGAQITSDPS EADNIVDKLK NILLRATSDG PSTPLIKNTT ALYKNEKGAN EVKNLEKYAS
190 200 210 220 230 240
VDISHILKKL PLNESFLKNP STKSFFLYNI DASIPEWKIT DTVSQLLGIK KWKDGNSLSL
250 260 270 280 290 300
IVNHKAKCGG LRFQSSELGE RFVSKISETL VTPKGLKRGV LLIDRFRIFI IPWSSGFSAA
310 320 330 340 350 360
SFGTNTAENI KLSLSLNKLI QLELGLSFPT KSTDNAKNDK KKTSKKVHKD RSKKSKPRAN
KLTI
References and
documentation are available.
Number of amino acids: 364
Molecular weight: 40924.53
Theoretical pI: 9.54
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 1811
Hydrogen H 2971
Nitrogen N 505
Oxygen O 537
Sulfur S 16
Formula: C
1811H
2971N
505O
537S
16
Total number of atoms: 5840
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28585
Abs 0.1% (=1 g/l) 0.698, assuming all pairs of Cys residues form cystines
Ext. coefficient 27960
Abs 0.1% (=1 g/l) 0.683, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.23
This classifies the protein as unstable.
Aliphatic index: 90.82
Grand average of hydropathicity (GRAVY): -0.412