ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNDIKQLLWN GELNVLVSID PSFLMKGSPR EIAVLRIRVP RETYLVNYMP LIWNKIKSFL 

        70         80         90        100        110        120 
SFDPLTDSEK YFWFEHNKTP IPWNYPVGVL FDCLAGKSAT FTTSFENQVK DVLTFLRIHL 

       130        140        150        160        170        180 
VMGDSLPPTI IPIASSKTQA EKFWFHQWKQ VCFILNGSSK AIMSLSVNEA RKFWGSVITR 

       190        200        210        220        230        240 
NFQDFIEISN KISSSRPRHI PLIIQTSRTS GTFRISQPTI SMTGVNPTLK DIEGDILDVK 

       250        260        270        280        290 
EGINGNDVMV ICQGIEIPWH MLLYDLYSKL RSFDGFLYIT LVPIKGGDKA SSEL 

References and documentation are available.

Number of amino acids: 294

Molecular weight: 33559.97

Theoretical pI: 8.65

Amino acid composition: 
Ala (A)   8	  2.7%
Arg (R)  12	  4.1%
Asn (N)  15	  5.1%
Asp (D)  15	  5.1%
Cys (C)   3	  1.0%
Gln (Q)   9	  3.1%
Glu (E)  13	  4.4%
Gly (G)  16	  5.4%
His (H)   5	  1.7%
Ile (I)  30	 10.2%
Leu (L)  28	  9.5%
Lys (K)  19	  6.5%
Met (M)   8	  2.7%
Phe (F)  18	  6.1%
Pro (P)  17	  5.8%
Ser (S)  28	  9.5%
Thr (T)  17	  5.8%
Trp (W)   8	  2.7%
Tyr (Y)   7	  2.4%
Val (V)  18	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1539
Hydrogen    H	      2395
Nitrogen    N	       391
Oxygen      O	       427
Sulfur      S	        11

Formula: C1539H2395N391O427S11
Total number of atoms: 4763

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54555
Abs 0.1% (=1 g/l)   1.626, assuming all pairs of Cys residues form cystines


Ext. coefficient    54430
Abs 0.1% (=1 g/l)   1.622, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.32
This classifies the protein as unstable.



Aliphatic index: 97.41

Grand average of hydropathicity (GRAVY): -0.020