ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNDMAKPLPT PPTAEIRKSR SNSPKKAQKT NLSPNKNQNN EKNVPRSNGR TKNEHNSMDD 

        70         80         90        100        110        120 
EEFEFFHQFS REKVKGVIHV ITAELKEKGP DVEFLMIPFR PEQTNDKLLT LLNQLFPLGN 

       130        140        150        160        170        180 
GQPVNEKKQL RIVSKADVWT LFQCLKYIWC RLPNSEIIGW KSYLEFKFRE EDKKFPRKSF 

       190        200        210        220        230        240 
LEIMPQCLAS PNHASIVYDF FDLIISISSN SRVNKMSARK ISKMCAIWAF SKQIPNSDIQ 

       250        260        270        280        290        300 
DYDFESAAMK SFAPNNSIQD GLDQWIPASD AMFHLLLAFL RSFVPQDLES AKLPRTLKSL 

       310        320        330        340        350        360 
LFNNQYPPRK STAYTSETIL TIPLVTLKTD VFSRKPWQLL ERCNDLLDFS DHDAFEARED 

       370        380        390        400        410        420 
YALLKSLFRK KNTVEGISRK MSQESRRLMK AMSTKHSTFQ PGWAPRECIE NISHLKECIE 

       430        440        450        460        470        480 
VKRLDIDDYF IWTWLSSLSF EQTSEKKKIF GRSIILEFEF DGFKKWVVFQ ECDITLDYNK 

       490        500        510        520        530        540 
KGQLKKKTSA QSPTTEKELP PDDFELEDPP LSKSPTLSQT YKKFQAEVPQ QSTVRRDSAP 

       550        560        570        580        590        600 
DNQGIYHTVI SKNALTKNKH NVNLHSFEHK ISKWNPLNNL RKKSGSNSSS SSFEEKSKDA 

       610        620        630        640        650        660 
PIREEYHTNK NHKSKKEERV LSQFSTLNPD EYQLPVIETG SSNFKIEIPE LMYEHDDDDS 

       670        680        690        700        710        720 
DKLKNSQKRA TDSAIEELNG MVEEMMINEP DDVKISITEA ETFESLTKFD QYKPSNITDD 

       730        740        750        760        770        780 
DLQSSHSSAV HSLKLSTNTN DSCADSSKYT ADRKLAEPRK ISEESKVNDD SSSYYSPNIN 

       790        800        810        820        830        840 
NLPASRMPSQ PTYSNSDSKK AFTNESRLNV LQGAVSPSQQ VTPKPYKNAP GDCVSPVQQK 

       850        860        870        880        890        900 
YYQNDRRNEM SPASAPVPPS AYSPARSPQF STNSAGFKQN TINVPVGYND PAHVLANQPH 

       910        920        930        940        950        960 
MTYRDQHNYP SHQQKQRPFQ NNIVPPELKS RNQRADASPI PQHMVPVKQG VPNLPSNVPL 

       970        980        990       1000       1010       1020 
YQQMERMNPN HQHPVNTYKV TQPPYHNNTT NAYGNSRAGN AHMLDGKWSN NPPQMVPKGV 

      1030       1040       1050       1060       1070       1080 
RPNQYPQQHV NRYSPQAQPV VPAEYYNGPP PMRAPPMMSH MVPAQEPIRY TAGANRRSFP 

      1090       1100       1110       1120       1130 
QGMQQNAYSV PAQPMGAVNS EFYLPEAPQG NKLHGNINKR QERKKLYDNI RSGNFGI 

References and documentation are available.

Number of amino acids: 1137

Molecular weight: 129971.19

Theoretical pI: 8.96

Amino acid composition: 
Ala (A)  61	  5.4%
Arg (R)  53	  4.7%
Asn (N)  89	  7.8%
Asp (D)  60	  5.3%
Cys (C)  10	  0.9%
Gln (Q)  67	  5.9%
Glu (E)  71	  6.2%
Gly (G)  33	  2.9%
His (H)  29	  2.6%
Ile (I)  52	  4.6%
Leu (L)  76	  6.7%
Lys (K)  95	  8.4%
Met (M)  30	  2.6%
Phe (F)  48	  4.2%
Pro (P)  95	  8.4%
Ser (S) 116	 10.2%
Thr (T)  51	  4.5%
Trp (W)  12	  1.1%
Tyr (Y)  38	  3.3%
Val (V)  51	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 131
Total number of positively charged residues (Arg + Lys): 148

Atomic composition:

Carbon      C	      5733
Hydrogen    H	      8935
Nitrogen    N	      1617
Oxygen      O	      1761
Sulfur      S	        40

Formula: C5733H8935N1617O1761S40
Total number of atoms: 18086

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   123245
Abs 0.1% (=1 g/l)   0.948, assuming all pairs of Cys residues form cystines


Ext. coefficient   122620
Abs 0.1% (=1 g/l)   0.943, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.18
This classifies the protein as unstable.



Aliphatic index: 62.28

Grand average of hydropathicity (GRAVY): -0.877