ProtParam
User-provided sequence:
10 20 30 40 50 60
MNDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVEQRES PLNNFSVAQC
70 80 90 100 110 120
QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KRQEEETMDF
130 140 150 160 170 180
RSGSPSDNSG AEEMEVALAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI
190 200 210 220 230 240
LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS
250 260 270 280 290 300
RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
310 320 330 340 350 360
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL
370 380 390 400 410 420
ILMEEIRFPR TLGPEAKSLL SGLLKKDPTQ RLGGGSEDAK EIMQHRFFAN IVWQDVYEKK
430 440 450 460 470 480
LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA
References and
documentation are available.
Number of amino acids: 480
Molecular weight: 55735.40
Theoretical pI: 5.63
Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 2490
Hydrogen H 3852
Nitrogen N 670
Oxygen O 739
Sulfur S 23
Formula: C
2490H
3852N
670O
739S
23
Total number of atoms: 7774
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65695
Abs 0.1% (=1 g/l) 1.179, assuming all pairs of Cys residues form cystines
Ext. coefficient 65320
Abs 0.1% (=1 g/l) 1.172, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.93
This classifies the protein as stable.
Aliphatic index: 71.69
Grand average of hydropathicity (GRAVY): -0.576