ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNDWHEFNAA IKSIYCNAEG DSSSIINRLV GLAMKSEDST FIEAVLVLKE NVSKVDKQLR 

        70         80         90        100        110        120 
FLWLTSTINS RFYPPIPISE ASPVSWNKTE YCAPGTEELQ RRYPGRAKLQ NEEDYSGGIE 

       130        140        150        160        170        180 
QCRDVPDCSL VASLINLRSK NLNLPLIKQI SSTKYHVNLS FNGSNKRLVT VDISQIPTSV 

       190        200        210        220        230        240 
DGKQLSLKSN DISDKIGELA LLLVSKGTYS TDGSNISIDT YRLSGFLPEI TQVNSYPFEK 

       250        260        270        280        290        300 
LWKFHKSNLC LMGAGTGNRS NDMIKPLVEN HDYSIIDITY DSRLVKLRDP RNSALNVEIS 

       310        320        330        340        350        360 
YEQYLKNFKQ LYLNWNQEKL FKRSQVLHFR YDTSRYNKFS IVADKPLFHL VNNSKVTETV 

       370        380        390        400        410        420 
WLLLESHLQD EGSQENRSVS FLNEAPECII CPIEPPVECG GNHIGLQLVK LRLDAETERL 

       430        440        450        460        470        480 
LYCYSTTNNN FSIHSFSVVK EICFQRLKDT KSLFAKVLFS FPYEIEGKAS FDTCNFFQNP 

       490        500        510        520        530        540 
TFELEVHSEQ DYQVLMDAAC ISTSSHDLIN IQVYYFNDYE LIKPIMFDNH YQPGQGLKQD 

       550        560        570        580        590        600 
VPILTNVKYM IVCSTYGPPA STEFELLASI RLSSSWRLIS GITLRSVNLI YGTYPYHCRN 

       610        620        630        640        650        660 
RFHWKETSDK LKIQMTLPTK KYSTNKLFIR VVPVESSARL RMRCNIFEPE SALCVYECQE 

       670        680        690        700        710        720 
YRTCPSGGIV IPDLEVSRTN IVVLMIERSV PISSCLPTEG QLDELELFVG SSQKIRIEKY 


SDDVIPK 

References and documentation are available.

Number of amino acids: 727

Molecular weight: 83123.84

Theoretical pI: 6.22

Amino acid composition: 
Ala (A)  23	  3.2%
Arg (R)  34	  4.7%
Asn (N)  46	  6.3%
Asp (D)  35	  4.8%
Cys (C)  19	  2.6%
Gln (Q)  27	  3.7%
Glu (E)  50	  6.9%
Gly (G)  29	  4.0%
His (H)  14	  1.9%
Ile (I)  54	  7.4%
Leu (L)  76	 10.5%
Lys (K)  45	  6.2%
Met (M)  10	  1.4%
Phe (F)  31	  4.3%
Pro (P)  34	  4.7%
Ser (S)  77	 10.6%
Thr (T)  36	  5.0%
Trp (W)   8	  1.1%
Tyr (Y)  33	  4.5%
Val (V)  46	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      3720
Hydrogen    H	      5827
Nitrogen    N	       983
Oxygen      O	      1117
Sulfur      S	        29

Formula: C3720H5827N983O1117S29
Total number of atoms: 11676

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94295
Abs 0.1% (=1 g/l)   1.134, assuming all pairs of Cys residues form cystines


Ext. coefficient    93170
Abs 0.1% (=1 g/l)   1.121, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.82
This classifies the protein as unstable.



Aliphatic index: 91.25

Grand average of hydropathicity (GRAVY): -0.288