ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNECEDTITR FCFRTTGINM SYSPERMSSY RRHFEGGLAA SSTLQVRVSS PSPTRGATRH 

        70         80         90        100        110        120 
RSASYSRSTG RRALSGTRKS RMTSSVSMGT LCLGMGFGGA GGAVDLDAAA AENQAFLSTR 

       130        140        150        160        170        180 
TSERKEMIAL NDRLAVYIEK VRSLEQSNKL LETEIDAIKG HHVKPSGLRL LYEEQLREMK 

       190        200        210        220        230        240 
KIADQMKIQR DLAVAAKDAM AGQLEMLKVK YEEALEARKK AELEIEAFRP DVDAATSARI 

       250        260        270        280        290        300 
ALEKRLENLE VELEFLQRIH KKEIEELMAQ IYGSVAKVDV AFSLPDLASA LKQIQAQYDS 

       310        320        330        340        350        360 
IAAQNLQDMD AWYKGKFQDL NNVSSRHMET ARSAREEIMG YKKEMQNKQR ELDALKNRNE 

       370        380        390        400        410        420 
ALLLMIKEAQ ENYRKEEEEL HTRIETLKEE LRSIKGKIAL LLREYQDLLN VKMSLEIEIT 

       430        440        450        460        470        480 
TYRKLIEGED SRLSSMVGGL SLMGGSVSSG LVSSVSAYSS AAAAAAAVSS AASEKMISSN 

       490        500        510        520        530 
STLNHNHHES VEEFTHEQAV EMTERKTVLI RTVKTDEDTI QKDTEERTIT ISGAADEDE 

References and documentation are available.

Number of amino acids: 539

Molecular weight: 60262.23

Theoretical pI: 5.85

Amino acid composition: 
Ala (A)  57	 10.6%
Arg (R)  40	  7.4%
Asn (N)  17	  3.2%
Asp (D)  24	  4.5%
Cys (C)   3	  0.6%
Gln (Q)  21	  3.9%
Glu (E)  61	 11.3%
Gly (G)  27	  5.0%
His (H)  11	  2.0%
Ile (I)  28	  5.2%
Leu (L)  53	  9.8%
Lys (K)  36	  6.7%
Met (M)  22	  4.1%
Phe (F)  10	  1.9%
Pro (P)   6	  1.1%
Ser (S)  53	  9.8%
Thr (T)  30	  5.6%
Trp (W)   1	  0.2%
Tyr (Y)  14	  2.6%
Val (V)  25	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 76

Atomic composition:

Carbon      C	      2587
Hydrogen    H	      4246
Nitrogen    N	       756
Oxygen      O	       845
Sulfur      S	        25

Formula: C2587H4246N756O845S25
Total number of atoms: 8459

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26485
Abs 0.1% (=1 g/l)   0.439, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.437, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.85
This classifies the protein as unstable.



Aliphatic index: 82.63

Grand average of hydropathicity (GRAVY): -0.513