ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNEEGCVPHN SDVVKQKSID QRAPLSGEPK CVICSRYGEY ICDETNDDVC SLECKQALLR 

        70         80         90        100        110        120 
RVDSARVFPA TDECFYVRDP GSSSHDAQLL RRKLDIHVQG QGSAVPPPVL TFTSCGLPPK 

       130        140        150        160        170        180 
LLLNLETAGY DFPTPIQMQA IPAALTGKSL LASADTGSGK TASFLVPIIS RCTTYHSEHP 

       190        200        210        220        230        240 
SDQRRNPLAM VLAPTRELCV QVEDQAKMLG KGLPFKTALV VGGDPMSGQL YRIQQGVELI 

       250        260        270        280        290        300 
IGTPGRVVDL LSKHTIELDN IMTFVLDEVD CMLQRGFRDQ VMQIFQALSQ PQVLLFSATI 

       310        320        330        340        350        360 
SREVEKVGGS LAKEIILVSI GNPNKPNKAV NQLAIWVDAK QKKQKLFDIL RSQNHFKPPA 

       370        380        390        400        410        420 
VVYVSSRVGA DLLANAITVV TGVKALSIHG EKPMKERRDV MGSFLGGEVP VLVSTGVLGR 

       430        440        450        460        470        480 
GVDLLVVRQV IVFDMPSTIK EYIHVIGRAS RMGEKGTAIV FVNEDDRNLF PDLVAALKSS 

       490        500 
GAAIPKELIN LTSREMHNKK RRVGY 

References and documentation are available.

Number of amino acids: 505

Molecular weight: 55217.97

Theoretical pI: 8.53

Amino acid composition: 
Ala (A)  34	  6.7%
Arg (R)  29	  5.7%
Asn (N)  16	  3.2%
Asp (D)  29	  5.7%
Cys (C)  11	  2.2%
Gln (Q)  25	  5.0%
Glu (E)  25	  5.0%
Gly (G)  37	  7.3%
His (H)  10	  2.0%
Ile (I)  29	  5.7%
Leu (L)  51	 10.1%
Lys (K)  30	  5.9%
Met (M)  13	  2.6%
Phe (F)  16	  3.2%
Pro (P)  30	  5.9%
Ser (S)  36	  7.1%
Thr (T)  23	  4.6%
Trp (W)   1	  0.2%
Tyr (Y)   9	  1.8%
Val (V)  51	 10.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2439
Hydrogen    H	      3974
Nitrogen    N	       684
Oxygen      O	       723
Sulfur      S	        24

Formula: C2439H3974N684O723S24
Total number of atoms: 7844

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19535
Abs 0.1% (=1 g/l)   0.354, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.342, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.56
This classifies the protein as unstable.



Aliphatic index: 97.80

Grand average of hydropathicity (GRAVY): -0.070