ProtParam
User-provided sequence:
10 20 30 40 50 60
MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP PLNNFSVAEC
70 80 90 100 110 120
QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWI RAIQMVANSL KQRGPGEDAM
130 140 150 160 170 180
DYKCGSPSDS STSEMMEVAV SKARAKVTMN DFDYLKLLGK GTFGKVILVR EKATGRYYAM
190 200 210 220 230 240
KILRKEVIIA KDEVAHTVTE SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGDLFFH
250 260 270 280 290 300
LSRERVFTED RARFYGAEIV SALEYLHSTD VVYRDIKLEN LMLDKDGHIK ITDFGLSKEG
310 320 330 340 350 360
ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHERLFE
370 380 390 400 410 420
LILMEEIRFP RTLGPEAKSL LAGLLKKDPK QRLGGGPSDA KEVMEHRFFL SINWQDVVQK
430 440 450 460 470 480
KLLPPFKPQV TSEVDTRYFD DEFTAQSITI TPPDRYDSLG SLELDQRTHF PQFSYSASIR
E
References and
documentation are available.
Number of amino acids: 481
Molecular weight: 55543.43
Theoretical pI: 5.97
Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 65
Atomic composition:
Carbon C 2489
Hydrogen H 3864
Nitrogen N 670
Oxygen O 729
Sulfur S 22
Formula: C
2489H
3864N
670O
729S
22
Total number of atoms: 7774
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67185
Abs 0.1% (=1 g/l) 1.210, assuming all pairs of Cys residues form cystines
Ext. coefficient 66810
Abs 0.1% (=1 g/l) 1.203, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.00
This classifies the protein as stable.
Aliphatic index: 77.01
Grand average of hydropathicity (GRAVY): -0.462