ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP PLNNFSVAEC 

        70         80         90        100        110        120 
QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWI RAIQMVANSL KQRGPGEDAM 

       130        140        150        160        170        180 
DYKCGSPSDS STSEMMEVAV SKARAKVTMN DFDYLKLLGK GTFGKVILVR EKATGRYYAM 

       190        200        210        220        230        240 
KILRKEVIIA KDEVAHTVTE SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGELFFH 

       250        260        270        280        290        300 
LSRERVFTED RARFYGAEIV SALEYLHSRD VVYRDIKLEN LMLDKDGHIK ITDFGLCKEG 

       310        320        330        340        350        360 
ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHERLFE 

       370        380        390        400        410        420 
LILMEEIRFP RTLGPEAKSL LAGLLKKDPK QRLGGGPSDA KEVMEHRFFL SINWQDVVQK 

       430        440        450        460        470        480 
KLLPPFKPQV TSEVDTRYFD DEFTAQSITI TPPDRYDSLG SLELDQRTHF PQFSYSASIR 


E 

References and documentation are available.

Number of amino acids: 481

Molecular weight: 55628.60

Theoretical pI: 6.08

Amino acid composition: 
Ala (A)  26	  5.4%
Arg (R)  35	  7.3%
Asn (N)  12	  2.5%
Asp (D)  31	  6.4%
Cys (C)   7	  1.5%
Gln (Q)  15	  3.1%
Glu (E)  41	  8.5%
Gly (G)  29	  6.0%
His (H)  11	  2.3%
Ile (I)  22	  4.6%
Leu (L)  44	  9.1%
Lys (K)  31	  6.4%
Met (M)  16	  3.3%
Phe (F)  26	  5.4%
Pro (P)  24	  5.0%
Ser (S)  27	  5.6%
Thr (T)  28	  5.8%
Trp (W)   7	  1.5%
Tyr (Y)  19	  4.0%
Val (V)  30	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      2492
Hydrogen    H	      3871
Nitrogen    N	       673
Oxygen      O	       727
Sulfur      S	        23

Formula: C2492H3871N673O727S23
Total number of atoms: 7786

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67185
Abs 0.1% (=1 g/l)   1.208, assuming all pairs of Cys residues form cystines


Ext. coefficient    66810
Abs 0.1% (=1 g/l)   1.201, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.24
This classifies the protein as stable.



Aliphatic index: 77.01

Grand average of hydropathicity (GRAVY): -0.463