ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNEVTCSITG DNPIHKINNG LGLKWNNLGK FSDFQTNDSA ARDARTIDYI FTNCQTGSSI 

        70         80         90        100        110        120 
GKIDFRAALP ADKSQHSGVS EKEFSRLENQ WSKEFSCFPK NKNADVTKPS RNKHEKRSAN 

       130        140        150        160        170        180 
LHNRYFAQYY STAYQQNRIY PCRISYNEHS SVSNGWEFQF KSIENQLLNE LKIENNVEEK 

       190        200        210        220        230        240 
TVGYEYVAEY EETIDFMHML SSVPQTYQFL KSNIYITERD PYKIGCVLMD NGSNLNEVVM 

       250        260        270        280        290        300 
AFEAAISQDP SHINAWLKLG IVNFENESES NGELALRNCL NLDPNNTIAL ENLAIHHINQ 

       310        320        330        340        350        360 
QNESESLKLF HKWILSKFSK VFQPSAGENK DSINKIPKKA HLAHILESLL NMGIEKKDQY 

       370        380        390        400        410        420 
DIYSVLSILY YSDQKIKQSQ KCLEFLLLEK PNNGTIWNRY GAILANTKSY HSAINAYNKC 

       430        440        450        460        470        480 
KQLRPNFTRV RYNLAIAYMN KGDYVKASKM LIEVILLRSK GYEHNKAKMQ NKFMQNLKNA 

       490        500        510 
LIASKNFDSL DLINGSHNTE SLISTLKAIY NKMD 

References and documentation are available.

Number of amino acids: 514

Molecular weight: 59064.80

Theoretical pI: 8.61

Amino acid composition: 
Ala (A)  32	  6.2%
Arg (R)  16	  3.1%
Asn (N)  56	 10.9%
Asp (D)  21	  4.1%
Cys (C)   8	  1.6%
Gln (Q)  22	  4.3%
Glu (E)  35	  6.8%
Gly (G)  20	  3.9%
His (H)  15	  2.9%
Ile (I)  39	  7.6%
Leu (L)  46	  8.9%
Lys (K)  46	  8.9%
Met (M)  11	  2.1%
Phe (F)  21	  4.1%
Pro (P)  13	  2.5%
Ser (S)  47	  9.1%
Thr (T)  18	  3.5%
Trp (W)   6	  1.2%
Tyr (Y)  25	  4.9%
Val (V)  17	  3.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 56
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2623
Hydrogen    H	      4086
Nitrogen    N	       722
Oxygen      O	       795
Sulfur      S	        19

Formula: C2623H4086N722O795S19
Total number of atoms: 8245

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70750
Abs 0.1% (=1 g/l)   1.198, assuming all pairs of Cys residues form cystines


Ext. coefficient    70250
Abs 0.1% (=1 g/l)   1.189, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.16
This classifies the protein as stable.



Aliphatic index: 80.31

Grand average of hydropathicity (GRAVY): -0.596