ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNFKILLPIC ALLTLTTFLL TIIATAGSTS NYKPITNIYI GDADISKINV TKVMPQVGPI 

        70         80         90        100        110        120 
LTVLGSALTA PNTTVDTIFG ALKAIASTEA LSPLLHLLSN AANTSATLSS LTQLAPMALL 

       130        140        150        160        170        180 
GTNTATTTTF SALDELLTTS KNTTELLDGF STLMSSMSTN TSSTSASLEN TVLTLLVDST 

       190        200        210        220        230        240 
NPIGTTESLI TLNNMTTEEK TKLSPVFELF ASSKNITATC DALETIMNST IPTSTVSSLF 

       250        260        270        280        290        300 
SSLKTSLAEG GNATETIMQL GSLVPSSLKP AVQAVVTLFD ETTSQNVTLS VLSTMIAENI 

       310        320        330        340        350        360 
TQSSSAKAAM GALTDLLNYT TNQTELLTSV ESLALSKEAA SSTNQLVALD EILSASANAS 

       370        380        390        400        410        420 
TVVSIIPTLE SQLANNTVLL KYVPYLFSLL AASSDPVSSF SSLVNITKWA ETNAATFMPM 

       430        440        450        460        470        480 
LKILNSAVNM TTITPEQLKE MTPSILEYLH IPVIYRLSIF TMCRAHLNRT MYSCSKSHAV 

       490        500        510        520        530        540 
QNMDFRSIVY NNIEGSDFKP YMDALNIGKD DLHLDGELQD RQHMYVPAVK AALAMNLMCI 

       550        560        570        580        590        600 
ITSFFLMVFL LLLSRRSVVS QKLWLALGFI SCWICIFSGL GSTIFSVILN MMKSGSKKDN 

       610        620        630        640        650        660 
YDVIISGSSP FYGLMWSGFV FAVLVFLCIA YCWWSSRKGA AIVEAEKAVQ ESDSTTSRII 

       670 
EEHESPIDAE KNFAR 

References and documentation are available.

Number of amino acids: 675

Molecular weight: 72697.86

Theoretical pI: 5.02

Amino acid composition: 
Ala (A)  60	  8.9%
Arg (R)  10	  1.5%
Asn (N)  38	  5.6%
Asp (D)  22	  3.3%
Cys (C)   9	  1.3%
Gln (Q)  15	  2.2%
Glu (E)  31	  4.6%
Gly (G)  23	  3.4%
His (H)   7	  1.0%
Ile (I)  47	  7.0%
Leu (L)  89	 13.2%
Lys (K)  28	  4.1%
Met (M)  25	  3.7%
Phe (F)  26	  3.9%
Pro (P)  23	  3.4%
Ser (S)  86	 12.7%
Thr (T)  77	 11.4%
Trp (W)   6	  0.9%
Tyr (Y)  14	  2.1%
Val (V)  39	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      3236
Hydrogen    H	      5218
Nitrogen    N	       806
Oxygen      O	      1012
Sulfur      S	        34

Formula: C3236H5218N806O1012S34
Total number of atoms: 10306

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54360
Abs 0.1% (=1 g/l)   0.748, assuming all pairs of Cys residues form cystines


Ext. coefficient    53860
Abs 0.1% (=1 g/l)   0.741, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.92
This classifies the protein as stable.



Aliphatic index: 104.22

Grand average of hydropathicity (GRAVY): 0.332