ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNGKPAFDKP KVELHVHLDG AIRLKTVLDV AKRRGISLPV SMEEELKELC TVNEPATLTE 

        70         80         90        100        110        120 
FLGKFSHFMH VIAGDREAIK RIAYEFVETK AKEGVIYVEA RYSPHFLANK GVEPLPWDQK 

       130        140        150        160        170        180 
PGDITPDDVV DLVNQGFKEG EQAFKTKARS ILCCMRHMPN WSMEVVELCK KFHKDGVVAI 

       190        200        210        220        230        240 
DLAGDESMNC ESYPGHKKAF EEAVRSNVHR TVHAGEVGPA SVVREAVEVL KAERIGHGYH 

       250        260        270        280        290        300 
TLEDQNLYKQ LLHQNMHFEM CPVSSRLTGA CEPDFTKHPL ITFKKDKANY SLNTDDPTIF 

       310        320        330        340        350 
NSTLNSDYEV VQKYMDFTEE EFKRLNINAA KSCFLPEKEK EKLLNQLYEA YGMRKSTSF 

References and documentation are available.

Number of amino acids: 359

Molecular weight: 40843.68

Theoretical pI: 6.13

Amino acid composition: 
Ala (A)  25	  7.0%
Arg (R)  15	  4.2%
Asn (N)  16	  4.5%
Asp (D)  19	  5.3%
Cys (C)   8	  2.2%
Gln (Q)   8	  2.2%
Glu (E)  36	 10.0%
Gly (G)  19	  5.3%
His (H)  15	  4.2%
Ile (I)  13	  3.6%
Leu (L)  29	  8.1%
Lys (K)  33	  9.2%
Met (M)  11	  3.1%
Phe (F)  18	  5.0%
Pro (P)  17	  4.7%
Ser (S)  18	  5.0%
Thr (T)  17	  4.7%
Trp (W)   2	  0.6%
Tyr (Y)  11	  3.1%
Val (V)  29	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      1817
Hydrogen    H	      2843
Nitrogen    N	       493
Oxygen      O	       540
Sulfur      S	        19

Formula: C1817H2843N493O540S19
Total number of atoms: 5712

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    27890
Abs 0.1% (=1 g/l)   0.683, assuming all pairs of Cys residues form cystines


Ext. coefficient    27390
Abs 0.1% (=1 g/l)   0.671, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.70
This classifies the protein as stable.



Aliphatic index: 76.02

Grand average of hydropathicity (GRAVY): -0.477