ProtParam
User-provided sequence:
10 20 30 40 50 60
MNGLVLGATG LCGGGFLRHA QEAPQFSKVY AILRRELPFP ATDKVVAIVE RDNSKWSQLI
70 80 90 100 110 120
TNEMNPQVLF TALATTRAAA GGLDKQYKID HDLNLQLAQA AKEKGCETIV LVSSAGAHPD
130 140 150 160 170 180
SRFGYMKMKG EIERDVIALD FKHIIILRPG PLLGERTNSK QSGFGGNLTA ALGTRVYRSR
190 200 210 220 230
FQRLLGYPVY GDEVGKVGVH LALNTSGKDK VQFVSSKDIL DISASLEKIA T
References and
documentation are available.
Number of amino acids: 231
Molecular weight: 25085.88
Theoretical pI: 9.30
Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1117
Hydrogen H 1803
Nitrogen N 315
Oxygen O 328
Sulfur S 6
Formula: C
1117H
1803N
315O
328S
6
Total number of atoms: 3569
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14565
Abs 0.1% (=1 g/l) 0.581, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.576, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.43
This classifies the protein as stable.
Aliphatic index: 95.45
Grand average of hydropathicity (GRAVY): -0.125