ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MNHWLAFLNK PESLKEQNSD CDQQGEMRHV TDGTLTKSPE SKPFRERRSQ TWIDSEVPTS 

        70         80         90        100        110        120 
TEKSNVQESI SSDIISKLSN RRSRRNRSES WAGSEASSPS GNISTLENAT EKNTLKSPNK 

       130        140        150        160        170        180 
FLQRGGLPTV GIGSQALSPA GKPSTLGNVS PGKFTTYKVH NSIEVNRFSS TPTKLLTNPH 

       190        200        210        220        230        240 
KVAAISNDEH YVVSNESLEE NIEVAHLENV FRSSKTPDEE QSEYMKLGEI RLSSSSYGGS 

       250        260        270        280        290        300 
ISKENSLPKV LDELQSQNEE IKALRQKLEE KDDRIQELEE LNSMNDAKLQ RIEDLQKEFH 

       310        320        330        340        350        360 
NERKAASKRL NIVQDRFRKE IKKIREEKIT DFQNKNASKK EKNEVTSAKT KCKAFSQRNI 

       370        380        390        400        410        420 
LVSELYRKQK QILNLQQEND KFLKDINESN NSIVKLRSEV EILKSNLQLS QDENKKLHDN 

       430        440        450        460        470        480 
GSFYEKRLND VYSYMQNLSL FEKDLGKFIL EEMKCGHSPS MFQNGFAKLY PDFQDIKNLE 

       490        500        510        520        530        540 
NMEQYKQLKG KIELLEKNDR IRLEKIISVF KLINERLHFM QQQHSHKIKY LQKEALTKEQ 

       550        560        570        580        590        600 
QFRLEKRRWH DILNLKEENF QKLKSELKGK LILSEKIQKN AEDKLNDYMN EHQEIVEKLQ 

       610        620        630        640        650        660 
NQALIASRWS TQIQESENTH KKITDELAGK QSEILKLEET ILSLKEDVFQ EKLNLKKLYG 

       670        680        690        700        710        720 
DPSTELNFET VGKSFPHITK EKYDSLGLDI LTDLTYVQSQ NLIKNLLIVL DIPLKTFLKI 

       730        740        750        760        770        780 
VPTIVIQLRC ELTLLTKFAN DLNLKVFGKQ LDFKSRRKVA MNEFLNNHDI AEVKHPLEYD 

       790 
LQALFKYFFS 

References and documentation are available.

Number of amino acids: 790

Molecular weight: 91740.10

Theoretical pI: 8.67

Amino acid composition: 
Ala (A)  26	  3.3%
Arg (R)  35	  4.4%
Asn (N)  59	  7.5%
Asp (D)  37	  4.7%
Cys (C)   4	  0.5%
Gln (Q)  48	  6.1%
Glu (E)  82	 10.4%
Gly (G)  25	  3.2%
His (H)  18	  2.3%
Ile (I)  49	  6.2%
Leu (L)  92	 11.6%
Lys (K)  91	 11.5%
Met (M)  11	  1.4%
Phe (F)  32	  4.1%
Pro (P)  21	  2.7%
Ser (S)  74	  9.4%
Thr (T)  34	  4.3%
Trp (W)   5	  0.6%
Tyr (Y)  17	  2.2%
Val (V)  30	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 119
Total number of positively charged residues (Arg + Lys): 126

Atomic composition:

Carbon      C	      4048
Hydrogen    H	      6528
Nitrogen    N	      1134
Oxygen      O	      1261
Sulfur      S	        15

Formula: C4048H6528N1134O1261S15
Total number of atoms: 12986

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53080
Abs 0.1% (=1 g/l)   0.579, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   0.576, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.17
This classifies the protein as unstable.



Aliphatic index: 83.91

Grand average of hydropathicity (GRAVY): -0.824